Evidence map›Paper›PMID 38807313›Full record

ArticleACS sensors2024

mRNA Display Pipeline for Protein Biosensor Construction.

Zhenling Cui, Cagla Ergun Ayva, Yi Jin Liew, Zhong Guo, Roxane Mutschler, Birgit Dreier, Maria M Fiorito, Patricia Walden, Christopher B Howard, Fernanda Ely and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS sensors, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. AApplied and environmental microbiology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Zhenling CuiARC Centre of Excellence in Synthetic Biology, Brisbane, Queensland 4001, Australia.
Cagla Ergun AyvaCentre for Agriculture and the Bioeconomy, Queensland University of Technology, Brisbane, Queensland 4001, Australia.
Yi Jin LiewCSIRO Health & Biosecurity, Westmead, New South Wales 2145,Australia.
Zhong GuoARC Centre of Excellence in Synthetic Biology, Brisbane, Queensland 4001, Australia.
Roxane MutschlerCentre for Agriculture and the Bioeconomy, Queensland University of Technology, Brisbane, Queensland 4001, Australia.ORCID 0009-0009-2376-4293
Birgit DreierDepartment of Biochemistry, University of Zurich, Winterthurerstrasse 190, Zurich CH-8057, Switzerland.
Maria M FioritoCentre for Agriculture and the Bioeconomy, Queensland University of Technology, Brisbane, Queensland 4001, Australia.
Patricia WaldenCentre for Agriculture and the Bioeconomy, Queensland University of Technology, Brisbane, Queensland 4001, Australia.
Christopher B HowardAustralian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Queensland 4072, Australia.ORCID 0000-0001-9797-8686
Fernanda ElyMaltexco S.A, Talagante 9680086,, Chile.
Andreas PlückthunDepartment of Biochemistry, University of Zurich, Winterthurerstrasse 190, Zurich CH-8057, Switzerland.ORCID 0000-0003-4191-5306
Carel PretoriusDepartment of Chemical Pathology, Pathology Queensland, Brisbane, Queensland 4006, Australia.
Jacobus Pj UngererDepartment of Chemical Pathology, Pathology Queensland, Brisbane, Queensland 4006, Australia.
Ashley M BucklePTNG Consulting, Melbourne, Victoria 3000, Australia.
Kirill AlexandrovARC Centre of Excellence in Synthetic Biology, Brisbane, Queensland 4001, Australia.ORCID 0000-0002-0957-6511

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Despite the significant potential of protein biosensors, their construction remains a trial-and-error process. The most obvious approach for addressing this is to utilize modular biosensor architectures where specificity-conferring modalities can be readily generated to recognize new targets. Toward this goal, we established a workflow that uses mRNA display-based selection of hyper-stable monobody domains for the target of choice or ribosome display to select equally stable DARPins. These binders were integrated into a two-component allosteric biosensor architecture based on a calmodulin-reporter chimera. This workflow was tested by developing biosensors for liver toxicity markers such as cytosolic aspartate aminotransferase, mitochondrial aspartate aminotransferase, and alanine aminotransferase 1. We demonstrate that our pipeline consistently produced >10

Indexed as

Biosensing TechniquesRNA, MessengerCalmodulinHumansCalmodulinRNA, MessengerDARPinsMonobodiesmRNA displayprotein biosensorsynthetic protein binding domain

Identifiers

PMID38807313
PMCPMC11218749

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.