Evidence map›Paper›PMID 38806892›Full record

ArticleEndocrine2024

Single-cell transcriptome analysis reveals the regulatory functions of islet exocrine cells after short-time obesogenic diet.

Qianqian Sun, Huiyu Tang, Huan Zhu, Yanyan Liu, Min Zhang, Chenghang Che, Bing Xiang, Shuang Wang

Abstract read
PubMed Publisher
In one paragraph

Article in Endocrine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Qianqian SunThe Center of Gerontology and Geriatrics, Sichuan University West China Hospital, Chengdu, Sichuan, China.
Huiyu TangThe Center of Gerontology and Geriatrics, Sichuan University West China Hospital, Chengdu, Sichuan, China.
Huan ZhuThe Center of Gerontology and Geriatrics, Sichuan University West China Hospital, Chengdu, Sichuan, China.
Yanyan LiuThe Center of Gerontology and Geriatrics, Sichuan University West China Hospital, Chengdu, Sichuan, China.
Min ZhangDepartment of Geriatrics, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan, China.
Chenghang CheThe Center of Gerontology and Geriatrics, Sichuan University West China Hospital, Chengdu, Sichuan, China.
Bing XiangDepartment of Hematology, Sichuan University West China Hospital, Chengdu, Sichuan, China. xiang7199@hotmail.com.
Shuang WangThe Center of Gerontology and Geriatrics, Sichuan University West China Hospital, Chengdu, Sichuan, China. wangs0211@wchscu.edu.cn.

Funding

Sichuan Province Science and Technology Support Program 2018FZ0046Sichuan Province Science and Technology Support Program 2021YFS0137
6 · The paper itself

Abstract

purposeThis study aims to investigate the functions of exocrine islet cell subtypes in the early stage of obesity induced by high-fat diet (HFD), which is accompanied with deterioration of the systemic insulin response and islet subpopulation abnormalities.

methodsIn this study, we analyzed published islet single-cell RNA sequencing (scRNA-seq) datasets from the early stage induced by HFD feeding. Bioinformatics tools such as findMarkers, Cellchat, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and Gene Ontology (GO) terms were applied to identify the different functions of exocrine cell clusters.

resultsA total of 26 cell clusters were obtained were identified from this dietary intervention model. Most proportions of cell subtypes were consistent between high-fat diet (HFD) and low-fat diet (LFD) groups, except for partial endocrine islet clusters and exocrine clusters. Most differentiated expression of genes in the HFD group was found in exocrine cluster. And we also found that the cell-cell interactions between ductal and endothelial cells were reduced in the HFD group, with the significant alteration in C17 (ductal) cluster. By further analyzing the co-expression regulatory network of transcription in the C17 cluster, we speculate that differentially expressed transcription factors affected the function of duct cells by affecting the expression of related genes in intercellular interaction networks, thereby promoting insulin resistance (IR) development.

conclusionOur results provide a reference for the function and regulatory mechanisms of exocrine cells in the obesity induced by HFD and probably influence the process of following insulin resistance.

Indexed as

Diet, High-FatGene Expression ProfilingIslets of LangerhansObesitySingle-Cell AnalysisAnimalsMiceSingle-Cell Gene Expression AnalysisTranscriptomeExocrine cellHigh-fat dietInsulin resistanceSingle-cell RNA sequencing

Identifiers

PMID38806892

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.