ArticleLight, science & applications2024
Ultra-high spatio-temporal resolution imaging with parallel acquisition-readout structured illumination microscopy (PAR-SIM).
Article in Light, science & applications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed.
- Effective point spread function modeling of photoacoustic remote sensing microscopy towards resolution enhancement.Photoacoustics · 2026Article
- Super-Resolution Structured Illumination Microscopy to Study Endocytosis and Cellular Nanoparticle Uptake.ACS nano · 2026Review
- Elucidating the Role of the Mixing Entropy in Equilibrated Nanoconfined Reactions.Entropy (Basel, Switzerland) · 2025Article
- Article
- Fully automated multicolour structured illumination module for super-resolution microscopy with two excitation colours.Communications engineering · 2025Article
- Fast reconstruction and optical-sectioning three-dimensional structured illumination microscopy.Innovation (Cambridge (Mass.)) · 2025Article
- Fast, faster, and the fastest structured illumination microscopy.Light, science & applications · 2024Article
- Ultra-high spatio-temporal resolution imaging with parallel acquisition-readout structured illumination microscopy (PAR-SIM).Light, science & applications · 2024Article
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
Structured illumination microscopy (SIM) has emerged as a promising super-resolution fluorescence imaging technique, offering diverse configurations and computational strategies to mitigate phototoxicity during real-time imaging of biological specimens. Traditional efforts to enhance system frame rates have concentrated on processing algorithms, like rolling reconstruction or reduced frame reconstruction, or on investments in costly sCMOS cameras with accelerated row readout rates. In this article, we introduce an approach to elevate SIM frame rates and region of interest (ROI) coverage at the hardware level, without necessitating an upsurge in camera expenses or intricate algorithms. Here, parallel acquisition-readout SIM (PAR-SIM) achieves the highest imaging speed for fluorescence imaging at currently available detector sensitivity. By using the full frame-width of the detector through synchronizing the pattern generation and image exposure-readout process, we have achieved a fundamentally stupendous information spatial-temporal flux of 132.9 MPixels · s
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.