ArticleJournal of advanced research2025
Engineering DMNT emission in cotton enhances direct and indirect defense against mirid bugs.
Article in Journal of advanced research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed.
- The salivary protein NlG8 from Nilaparvata lugens induces both direct and indirect resistance in host rice plants.The New phytologist · 2026Article
- Chemical Signaling and Integrated Strategies for Biological Control in Crop Agroecosystems.Insects · 2026Review
- Monitoring and Volatile Profiling of Fruit Crops as Host Plants of the Polyphagous Brown Marmorated Stink BugInsects · 2026Article
- Harnessing semiochemicals for parasitoid-based biological control: from laboratory identification to field applications.Crop health · 2025Review
- Pangenome Identification and Analysis of Terpene Synthase Gene Family Members inInternational journal of molecular sciences · 2024Article
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9 authors.
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Abstract
introductionAs an important herbivore-induced plant volatile, (3E)-4,8-dimethyl-1,3,7-nonatriene (DMNT) is known for its defensive role against multiple insect pests, including attracting natural enemies. A terpene synthase (GhTPS14) and two cytochrome P450 (GhCYP82L1, GhCYP82L2) enzymes are involved in the de novo synthesis of DMNT in cotton. We conducted a study to test the potential of manipulating DMNT-synthesizing enzymes to enhance plant resistance to insects.
objectivesTo manipulate DMNT emissions in cotton and generate cotton lines with increased resistance to mirid bug Apolygus lucorum.
methodsBiosynthesis and emission of DMNT by cotton plants were altered using CRISPR/Cas9 and overexpression approaches. Dynamic headspace sampling and GC-MS analysis were used to collect, identify and quantify volatiles. The attractiveness and suitability of cotton lines against mirid bug and its parasitoid Peristenus spretus were evaluated through various assays.
resultsNo DMNT emission was detected in knockout CAS-L1L2 line, where both GhCYP82L1 and GhCYP82L2 were knocked out. In contrast, gene-overexpressed lines released higher amounts of DMNT when infested by A. lucorum. At the flowering stage, L114 (co-overexpressing GhCYP82L1 and GhTPS14) emitted 10-15-fold higher amounts than controls. DMNT emission in overexpressed transgenic lines could be triggered by methyl jasmonate (MeJA) treatment. Apolygus lucorum and its parasitoid were far less attracted to the double edited CAS-L1L2 plants, however, co-overexpressed line L114 significantly attracted bugs and female wasps. A high dose of DMNT, comparable to the emission of L114, significantly inhibited the growth of A. lucorum, and further resulted in higher mortalities.
conclusionTurning down DMNT emission attenuated the behavioral preferences of A. lucorum to cotton. Genetically modified cotton plants with elevated DMNT emission not only recruited parasitoids to enhance indirect defense, but also formed an ecological trap to kill the bugs. Therefore, manipulation of DMNT biosynthesis and emission in plants presents a promising strategy for controlling mirid bugs.
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