ArticleProceedings of the National Academy of Sciences of the United States of America2024
Deep sequencing of Phox2a nuclei reveals five classes of anterolateral system neurons.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed.
- Deep Cellular and Spatial Profiling of the Mouse Spinal Cord Reveals Sex-Specific Neuron Types and the Ascending Projection Neuron Repertoire.bioRxiv : the preprint server for biology · 2026Article
- Article
- NaThe Journal of comparative neurology · 2026Article
- Evidence for chronological diversification of spinal neuron subtypes by a shared sequence of transcription factors.Science advances · 2026Article
- Cellular and molecular basis of neuropathic pain.RMD open · 2026Review
- Integrative epigenomic and transcriptomic profiling reveals organ-specific and coordinated cold stress responses in the brain and gill of Nile tilapia.Stress biology · 2026Article
- Suppression of neuropathic pain hypersensitivity by TRPM8 is mediated by mGluR group II and III receptors acting differentially on distinct nociceptor inputs to spinal cord.Frontiers in pharmacology · 2026Article
- Central neural circuits underlying itch sensation.Nature reviews. Neuroscience · 2025Review
- Phox2a in Lateral Spinal Nucleus Tac1-Positive Neurons Mediates Histamine-Independent Acute Itch.CNS neuroscience & therapeutics · 2025Article
- A molecular map of the human spinal dorsal and ventral horn defines arrangement of neuronal types and glial sex differences.bioRxiv : the preprint server for biology · 2025Article
- Differential modification of ascending spinal outputs in acute and chronic pain states.Neuron · 2025Article
- Anatomical characterisation of somatostatin-expressing neurons belonging to the anterolateral system.Scientific reports · 2025Article
- A Viral Labelling Study of Spinal Trigeminal Nucleus Caudalis Projection Neurons Targeting the Parabrachial Nucleus.Journal of neurochemistry · 2025Article
- An electrophysiologist's guide to dorsal horn excitability and pain.Frontiers in cellular neuroscience · 2025Review
- Lateral lamina V projection neuron axon collaterals connect sensory processing across the dorsal horn of the mouse spinal cord.Scientific reports · 2024Article
- Deep sequencing of Phox2a nuclei reveals five classes of anterolateral system neurons.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- VLK drives extracellular phosphorylation of EphB2 to govern the EphB2-NMDAR interaction and injury-induced pain.bioRxiv : the preprint server for biology · 2024Article
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Abstract
The anterolateral system (ALS) is a major ascending pathway from the spinal cord that projects to multiple brain areas and underlies the perception of pain, itch, and skin temperature. Despite its importance, our understanding of this system has been hampered by the considerable functional and molecular diversity of its constituent cells. Here, we use fluorescence-activated cell sorting to isolate ALS neurons belonging to the Phox2a-lineage for single-nucleus RNA sequencing. We reveal five distinct clusters of ALS neurons (ALS1-5) and document their laminar distribution in the spinal cord using in situ hybridization. We identify three clusters of neurons located predominantly in laminae I-III of the dorsal horn (ALS1-3) and two clusters with cell bodies located in deeper laminae (ALS4 and ALS5). Our findings reveal the transcriptional logic that underlies ALS neuronal diversity in the adult mouse and uncover the molecular identity of two previously identified classes of projection neurons. We also show that these molecular signatures can be used to target groups of ALS neurons using retrograde viral tracing. Overall, our findings provide a valuable resource for studying somatosensory biology and targeting subclasses of ALS neurons.
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