ArticleNature plants2024
Removal of the large inverted repeat from the plastid genome reveals gene dosage effects and leads to increased genome copy number.
Article in Nature plants, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed.
- Characterisation of the chloroplast genome of Macrotyloma species: comparative analysis and phylogenomic insights.Scientific reports · 2026Article
- Pan-Plastome Evolution and Metabolite Variation Provide Insights to Conservation of the Tibetan Medicinal PlantPlants (Basel, Switzerland) · 2026Article
- Comparative analysis of six new chloroplast genomes in Platanthera (Orchidaceae) enhances understanding of its diversification.Scientific reports · 2026Article
- The evolutionary path of widespread IR heteroplasmy of chloroplast genomes in the context of Rhodophyta phylogeny.Journal of molecular evolution · 2026Article
- Editing and synthetic engineering of chloroplast genomes.Plant communications · 2026Review
- Chloroplast genome comparison of Valeriana species with sequence variation, selective pressure, and divergence analysis.PloS one · 2026Article
- The complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2026Article
- Editorial: Evolutionary dynamics, functional variation and application of plant organellar genomes.Frontiers in plant science · 2026Article
- Assembly and comparative analysis of the complete mitochondrial and chloroplast genomes of the medicinal plant Vincetoxicum Pycnostelma.BMC genomics · 2025Article
- Sequence dynamics and plastome evolution: decoding the complete chloroplast genome of Oenothera drummondii and comparative analysis within Oenothera (Onagraceae).Functional & integrative genomics · 2025Article
- Wheat chloroplast pangenome reveals frequent intramolecular recombination in the inverted repeat regions.BMC plant biology · 2025Article
- Article
- Development of chloroplast transformation for five species in the genus Nicotiana.The Plant journal : for cell and molecular biology · 2025Article
- Assembly-dependent translational feedback regulation of photosynthetic proteins in land plants.Nature plants · 2025Article
- Beyond conservation: the landscape of chloroplast genome rearrangements in angiosperms.The New phytologist · 2025Review
- Comparative plastome analysis reveals evolutionary dynamics and codon usage patterns in Bidens (Asteraceae).Functional & integrative genomics · 2025Article
- Reminiscent of the pre-diatom? A hitherto undescribed scaly bolidophyte Lepidoparma frigida gen. et sp. nov. in a new order Lepidoparmales based on morphology, phylogeny, and ecology.Journal of phycology · 2025Article
- Comparative and evolutionary analysis of chloroplast genomes from five rare Styrax species.BMC genomics · 2025Article
- Chloroplast genomic insights into adaptive evolution and rapid radiation in the genus Passiflora (Passifloraceae).BMC plant biology · 2025Article
- Dispersed repeats and inverted repeat expansion drive major plastomic rearrangements inFrontiers in plant science · 2025Article
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Abstract
The chloroplast genomes of most plants and algae contain a large inverted repeat (IR) region that separates two single-copy regions and harbours the ribosomal RNA operon. We have addressed the functional importance of the IR region by removing an entire copy of the 25.3-kb IR from the tobacco plastid genome. Using plastid transformation and subsequent selectable marker gene elimination, we precisely excised the IR, thus generating plants with a substantially reduced plastid genome size. We show that the lack of the IR results in a mildly reduced plastid ribosome number, suggesting a gene dosage benefit from the duplicated presence of the ribosomal RNA operon. Moreover, the IR deletion plants contain an increased number of plastid genomes, suggesting that genome copy number is regulated by measuring total plastid DNA content rather than by counting genomes. Together, our findings (1) demonstrate that the IR can enhance the translation capacity of the plastid, (2) reveal the relationship between genome size and genome copy number, and (3) provide a simplified plastid genome structure that will facilitate future synthetic biology applications.
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