Evidence map›Paper›PMID 38800747›Full record

ArticleFrontiers in microbiology2024

Development and biological characterization of an infectious cDNA clone of NADC34-like PRRSV.

Yafang Lin, Lujia Zhou, Changguang Xiao, Zongjie Li, Ke Liu, Beibei Li, Donghua Shao, Yafeng Qiu, Zhiyong Ma, Jianchao Wei

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yafang Lin *Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Lujia Zhou *Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Changguang XiaoShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Zongjie LiShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Ke LiuShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Beibei LiShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Donghua ShaoShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Yafeng QiuShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Zhiyong MaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Jianchao WeiShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Porcine Reproductive and Respiratory Syndrome virus (PRRSV) causes high abortion rates in gestating sows and stillbirths, as well as high piglet mortality, seriously jeopardizing the pig industry in China and worldwide. Methods: In this study, an infectious clone containing the full-length genome of NADC34-like PRRSV was constructed for the first time using reverse genetic techniques. The gene was amplified segmentally onto a plasmid, transfected into BHK-21 cells, and the transfected supernatant was harvested and transfected into PAM cells, which showed classical cytopathic effects (CPE). Results: The virus rJS-KS/2021 was successfully rescued which could be demonstrated by Western Blot and indirect immunofluorescence assays. Its growth curve was similar to the original strain. Replace the 5'UTR and 3'UTR of rJS-KS/2021 with 5'UTR and 3'UTR of HP-PRRSV (strain SH1) also failed to propagate on MARC-145. Discussion: In this study, an infectious clone of NADC34-like was constructed by reverse genetics, replacing the UTR and changing the cellular tropism of the virus. These findings provide a solid foundation for studying the recombination of different PRRSVs and the adaption of PRRSVs on MARC-145 in the future.

Indexed as

biological characteristicsinfectious clonesNADC34-likeporcine reproductive and respiratory syndrome virusrecombinant virus

Identifiers

PMID38800747
PMCPMC11123230

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.