Evidence map›Paper›PMID 38799348›Full record

ArticleACS omega2024

Single-Cell Sensitive Colorimetric pH Detection Based on Microscope Ratiometric Grayscale.

Shuo Zhou, Anqi Li, Linying Zhao, Yanwen Li, Xiaolong Xu, Yongdong Jin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS omega, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Shuo ZhouSchool of Environmental and Chemical Engineering, Institute of Carbon Peaking and Carbon Neutralization, Wuyi University, Jiangmen 529020, PR China.
Anqi LiSchool of Environmental and Chemical Engineering, Institute of Carbon Peaking and Carbon Neutralization, Wuyi University, Jiangmen 529020, PR China.
Linying ZhaoSchool of Environmental and Chemical Engineering, Institute of Carbon Peaking and Carbon Neutralization, Wuyi University, Jiangmen 529020, PR China.
Yanwen LiSchool of Environmental and Chemical Engineering, Institute of Carbon Peaking and Carbon Neutralization, Wuyi University, Jiangmen 529020, PR China.
Xiaolong XuSchool of Environmental and Chemical Engineering, Institute of Carbon Peaking and Carbon Neutralization, Wuyi University, Jiangmen 529020, PR China.
Yongdong JinGuangdong Key Laboratory of Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Shenzhen University Medical School, Shenzhen University, Shenzhen 518060, PR China.ORCID https://orcid.org/0000-0002-0466-9065

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rapid and accurate identification of the intracellular pH is critical in the field of biomedicine. In this work, we effectively identified and quantified the intracellular pH and its distribution at the single-cell level using an image sensor based on an ordinary bright-field optical microscope that divided the cell staining images into their red (R) and blue (B) channels. The grayscale of the R and B channels was subjected to a ratiometric operation to generate ratiometric grayscale cell images of the microscope. A standard curve of pH against ratiometric grayscale curve was then obtained by incubating HeLa cells at pH 6.00-7.60 in a high concentration K

Identifiers

PMID38799348
PMCPMC11112558

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.