Evidence map›Paper›PMID 38797697›Full record

ArticleCell structure and function2024

A high-resolution phase-contrast microscopy system for label-free imaging in living cells.

Kentaro Shimasaki, Yuko Okemoto-Nakamura, Kyoko Saito, Masayoshi Fukasawa, Kaoru Katoh, Kentaro Hanada

Abstract read
In one paragraph

Article in Cell structure and function, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Kentaro ShimasakiDepartment of Biochemistry and Cell Biology, National Institute of Infectious Diseases.
Yuko Okemoto-NakamuraDepartment of Biochemistry and Cell Biology, National Institute of Infectious Diseases.
Kyoko SaitoDepartment of Biochemistry and Cell Biology, National Institute of Infectious Diseases.
Masayoshi FukasawaDepartment of Biochemistry and Cell Biology, National Institute of Infectious Diseases.
Kaoru KatohBiomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST).
Kentaro HanadaCenter for Quality Management Systems, National Institute of Infectious Diseases.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell biologists have long sought the ability to observe intracellular structures in living cells without labels. This study presents procedures to adjust a commercially available apodized phase-contrast (APC) microscopy system for better visualizing the dynamic behaviors of various subcellular organelles in living cells. By harnessing the versatility of this technique to capture sequential images, we could observe morphological changes in cellular geometry after virus infection in real time without probes or invasive staining. The tune-up APC microscopy system is a highly efficient platform for simultaneously observing the dynamic behaviors of diverse subcellular structures with exceptional resolution.

Indexed as

Microscopy, Phase-ContrastAnimalsHeLa CellsHumansOrganellesapodized phase contrastlabel-free imagingorganelle dynamicsvirus infections

Identifiers

PMID38797697
PMCPMC11496782

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.