Evidence map›Paper›PMID 38796812›Full record

ArticleJournal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry2024

Catalytic role of histidine-114 in the hydrolytic dehalogenation of chlorothalonil by Pseudomonas sp. CTN-3.

Grayson Gerlich, Callie Miller, Xinhang Yang, Karla Diviesti, Brian Bennett, Judith Klein-Seetharaman, Richard C Holz

Abstract read
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Article in Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Grayson GerlichContribution from the Quantitative Biosciences and Engineering Program, Colorado School of Mines, Golden, CO, 80401, USA.
Callie MillerDepartment of Chemistry, Colorado School of Mines, Golden, CO, 80401, USA.
Xinhang YangDepartment of Chemistry, Marquette University, Milwaukee, WI, 53233, USA.
Karla DiviestiContribution from the Quantitative Biosciences and Engineering Program, Colorado School of Mines, Golden, CO, 80401, USA.
Brian BennettDepartment of Physics, Marquette University, 1420 W. Clybourn St, Milwaukee, WI, 53233, USA. brian.bennett@marquette.edu.
Judith Klein-SeetharamanSchool of Molecular Sciences, Arizona State University, Phoenix, AZ, 85004, USA. judith.klein-seetharaman@asu.edu.
Richard C HolzDepartment of Chemistry, Colorado School of Mines, Golden, CO, 80401, USA. rholz@mines.edu.

Funding

TR&D Project 3P41EB001980 · NIBIB · MEDICAL COLLEGE OF WISCONSIN · PI KLUG, CANDICE S · 2003 to 2017
$16.3M
National Science Foundation CHE-1532168National Science Foundation CHE-1808711National Science Foundation CHE-2003861NIH HHS P41-EB001980
6 · The paper itself

Abstract

Chlorothalonil (2,4,5,6-tetrachloroisophthalonitrile; TPN) is an environmentally persistent fungicide that sees heavy use in the USA and is highly toxic to aquatic species and birds, as well as a probable human carcinogen. The chlorothalonil dehalogenase from Pseudomonas sp. CTN-3 (Chd, UniProtKB C9EBR5) degrades TPN to its less toxic 4-OH-TPN analog making it an exciting candidate for the development of a bioremediation process for TPN; however, little is currently known about its catalytic mechanism. Therefore, an active site residue histidine-114 (His114) which forms a hydrogen bond with the Zn(II)-bound water/hydroxide and has been suggested to be the active site acid/base, was substituted by an Ala residue. Surprisingly, Chd

Indexed as

HistidineNitrilesPseudomonasBiocatalysisCatalytic DomainFungicides, IndustrialHalogenationHydrolasesHydrolysisFungicides, IndustrialHistidineHydrolasesNitrilestetrachloroisophthalonitrileChlorothalonilDehalogenaseElectron paramagnetic resonanceMetalloenzymeMolecular dynamicsZinc

Identifiers

PMID38796812

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.