Evidence map›Paper›PMID 38796562›Full record

ArticleMolecular biology reports2024

Expression profile of viability and stress response genes as a result of resveratrol supplementation in vitrified and in vitro produced cattle embryos.

Yasser H A Saber, Sally Ibrahim, Karima Gh M Mahmoud, Wahid M Ahmed, Refaat S A Ragab, Adel A M Seida

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Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Recent Advances and Emerging Applications in Embryo Vitrification for Livestock.Reproduction in domestic animals = Zuchthygiene · 2026
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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yasser H A SaberDepartment of Animal Reproduction and A.I, Veterinary Research Institute, National Research Centre, Dokki, Giza, Egypt. Yasserhussein_2011@yahoo.com.
Sally IbrahimDepartment of Animal Reproduction and A.I, Veterinary Research Institute, National Research Centre, Dokki, Giza, Egypt.
Karima Gh M MahmoudDepartment of Animal Reproduction and A.I, Veterinary Research Institute, National Research Centre, Dokki, Giza, Egypt.
Wahid M AhmedDepartment of Animal Reproduction and A.I, Veterinary Research Institute, National Research Centre, Dokki, Giza, Egypt.
Refaat S A RagabDepartment of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Adel A M SeidaDepartment of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.ORCID https://orcid.org/0000-0002-3002-7621

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundResveratrol, a potent antioxidant, is known to induce the up-regulation of the internal antioxidant system. Therefore, it holds promise as a method to mitigate cryopreservation-induced injuries in bovine oocytes and embryos. This study aimed to (i) assess the enhancement in the quality of in vitro produced bovine embryos following resveratrol supplementation and (ii) monitor changes in the expression of genes associated with oxidative stress (GPX4, SOD, CPT2, NFE2L2), mitochondrial function (ATP5ME), endoplasmic reticulum function (ATF6), and embryo quality (OCT4, DNMT1, CASP3, ELOVL5). METHODS AND

resultsThree groups of in vitro bovine embryos were cultured with varying concentrations of resveratrol (0.01, 0.001, and 0.0001 µM), with a fourth group serving as a control. Following the vitrification process, embryos were categorized as either good or poor quality. Blastocysts were then preserved at - 80 °C for RNA isolation, followed by qRT-PCR analysis of selected genes. The low concentrations of resveratrol (0.001 µM, P < 0.05 and 0.0001 µM, P < 0.01) significantly improved the blastocyst rate compared to the control group. Moreover, the proportion of good quality vitrified embryos increased significantly (P < 0.05) in the groups treated with 0.001 and 0.0001 µM resveratrol compared to the control group. Analysis of gene expression showed a significant increase in OCT4 and DNMT1 transcripts in both good and poor-quality embryos treated with resveratrol compared to untreated embryos. Additionally, CASP3 expression was decreased in treated good embryos compared to control embryos. Furthermore, ELOVL5 and ATF6 transcripts were down-regulated in treated good embryos compared to the control group. Regarding antioxidant-related genes, GPX4, SOD, and CPT2 transcripts increased in the treated embryos, while NFE2L2 mRNA decreased in treated good embryos compared to the control group.

conclusionsResveratrol supplementation at low concentrations effectively mitigated oxidative stress and enhanced the cryotolerance of embryos by modulating the expression of genes involved in oxidative stress response.

Indexed as

AntioxidantsBlastocystCryopreservationOxidative StressResveratrolVitrificationAnimalsCattleEmbryo Culture TechniquesEmbryo, MammalianEmbryonic DevelopmentFemaleFertilization in VitroGene Expression Regulation, DevelopmentalOocytesAntioxidantsResveratrolAntioxidantsGene expressionIn vitro bovine embryosOxidative stressResveratrolVitrification

Identifiers

PMID38796562

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.