Evidence map›Paper›PMID 38796461›Full record

ArticleAnnals of clinical microbiology and antimicrobials2024

Comparison of testing methods assessing the in vitro efficacy of the combination of aztreonam with avibactam on multidrug-resistant Gram-negative bacilli.

Corentin Deckers, Florian Bélik, Olivier Denis, Pierre Bogaerts, Isabel Montesinos, Catherine Berhin, Warda Bouchahrouf, Martin Hoebeke, Stephanie Evrard, Nicolas Gilliard and 2 more

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Annals of clinical microbiology and antimicrobials, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Whole-genome analysis of NDM-producingEmerging microbes & infections · 2025
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Corentin Deckers *National Reference Center for Antibiotic-Resistant Gram-Negative Bacilli, CHU UCL Namur and Université Catholique de Louvain, Avenue Gaston Therasse, 1, 5530, Yvoir, Belgium. corentin.deckers@uclouvain.be.
Florian Bélik *National Reference Center for Antibiotic-Resistant Gram-Negative Bacilli, CHU UCL Namur and Université Catholique de Louvain, Avenue Gaston Therasse, 1, 5530, Yvoir, Belgium.
Olivier DenisNational Reference Center for Antibiotic-Resistant Gram-Negative Bacilli, CHU UCL Namur and Université Catholique de Louvain, Avenue Gaston Therasse, 1, 5530, Yvoir, Belgium.
Pierre BogaertsNational Reference Center for Antibiotic-Resistant Gram-Negative Bacilli, CHU UCL Namur and Université Catholique de Louvain, Avenue Gaston Therasse, 1, 5530, Yvoir, Belgium.
Isabel MontesinosNational Reference Center for Antibiotic-Resistant Gram-Negative Bacilli, CHU UCL Namur and Université Catholique de Louvain, Avenue Gaston Therasse, 1, 5530, Yvoir, Belgium.
Catherine BerhinNational Reference Center for Antibiotic-Resistant Gram-Negative Bacilli, CHU UCL Namur and Université Catholique de Louvain, Avenue Gaston Therasse, 1, 5530, Yvoir, Belgium.
Warda BouchahroufNational Reference Center for Antibiotic-Resistant Gram-Negative Bacilli, CHU UCL Namur and Université Catholique de Louvain, Avenue Gaston Therasse, 1, 5530, Yvoir, Belgium.
Martin HoebekeNational Reference Center for Antibiotic-Resistant Gram-Negative Bacilli, CHU UCL Namur and Université Catholique de Louvain, Avenue Gaston Therasse, 1, 5530, Yvoir, Belgium.
Stephanie EvrardNational Reference Center for Antibiotic-Resistant Gram-Negative Bacilli, CHU UCL Namur and Université Catholique de Louvain, Avenue Gaston Therasse, 1, 5530, Yvoir, Belgium.
Nicolas GilliardNational Reference Center for Antibiotic-Resistant Gram-Negative Bacilli, CHU UCL Namur and Université Catholique de Louvain, Avenue Gaston Therasse, 1, 5530, Yvoir, Belgium.
Merve OkurNational Reference Center for Antibiotic-Resistant Gram-Negative Bacilli, CHU UCL Namur and Université Catholique de Louvain, Avenue Gaston Therasse, 1, 5530, Yvoir, Belgium.
Te-Din HuangNational Reference Center for Antibiotic-Resistant Gram-Negative Bacilli, CHU UCL Namur and Université Catholique de Louvain, Avenue Gaston Therasse, 1, 5530, Yvoir, Belgium.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAztreonam-avibactam (ATM-AVI) combination shows promising effectiveness on most carbapenemase-producing Gram-negatives, yet standardized antibiotic susceptibility testing (AST) methods for evaluating the combination in clinical laboratories is lacking. We aimed to evaluate different ATM-AVI AST approaches.

methods96 characterized carbapenem-resistant clinical isolates belonging to 9 Enterobacterales (EB; n = 80) and P. aeruginosa (PA; n = 16) species, including 90 carbapenemase producers and 72 strains resistant to both CAZ-AVI and ATM, were tested. Paper disk elution (DE; Bio-Rad) and E-test gradient strips stacking (SS; bioMérieux) were performed for the ATM + CAZ-AVI combination. MIC Test Strip (MTS; Liofilchem) was evaluated for ATM-AVI MIC determination. Results were interpreted applying ATM clinical breakpoints of the EUCAST guidelines and compared to the broth microdilution method (Sensititre, Thermofisher).

resultsAccording to broth microdilution method, 93% of EB and 69% of PA were tested susceptible to ATM-AVI. The synergistic effect of ATM-AVI was of 95% for EB, but of only 17% for PA. The MTS method yielded higher categorical and essential agreement (CA/EA) rates for both EB (89%/91%) and PA (94%/94%) compared to SS, where the rates were 87%/83% for EB and 81%/81% for PA. MTS and SS yielded 2 and 3 major discrepancies, respectively, while 3 very major discrepancies each were observed for both methods. Concerning the DE method, CA reached 91% for EB and 81% for PA, but high number of very major discrepancies were observed for EB (n = 6; 8%) and for PA (n = 3; 19%).

conclusionsThe ATM-AVI association displayed excellent in vitro activity against highly resistant clinical Enterobacterales strains. MTS method offers accurate ATM-AVI AST results, while the SS method might serve as better alternative then DE method in assessing the efficacy of ATM + CAZ-AVI combination. However, further investigation is needed to confirm the methods' ability to detect ATM-AVI resistance.

Indexed as

Anti-Bacterial AgentsAzabicyclo CompoundsAztreonamDrug Resistance, Multiple, BacterialGram-Negative BacteriaMicrobial Sensitivity TestsBacterial Proteinsbeta-LactamasesDrug CombinationsEnterobacteriaceaeGram-Negative Bacterial InfectionsHumansPseudomonas aeruginosaAnti-Bacterial AgentsavibactamAzabicyclo CompoundsAztreonamBacterial Proteinsbeta-LactamasescarbapenemaseDrug CombinationsAntibiotic combinationAntibiotic susceptibility testingAztreonam-avibactamCarbapenemaseMetallo-β-lactamase

Identifiers

PMID38796461
PMCPMC11127339

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.