Evidence map›Paper›PMID 38795199›Full record

ArticleMikrochimica acta2024

Based ATP-gating mechanism for detection of alkaline phosphatase in single-glass micropipettes functionalized by three-dimensional DNA network.

Juanjuan Zheng, Jinzheng Zhang, Shiwei Xu, Yueyue Feng, Liying Huang, Guofeng Wang, Nannan Liu

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In one paragraph

Article in Mikrochimica acta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Juanjuan ZhengKey Lab of Biohealth Materials and Chemistry of Wenzhou, College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou, Zhejiang, 325027, People's Republic of China.
Jinzheng ZhangKey Lab of Biohealth Materials and Chemistry of Wenzhou, College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou, Zhejiang, 325027, People's Republic of China.
Shiwei XuKey Lab of Biohealth Materials and Chemistry of Wenzhou, College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou, Zhejiang, 325027, People's Republic of China.
Yueyue FengKey Lab of Biohealth Materials and Chemistry of Wenzhou, College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou, Zhejiang, 325027, People's Republic of China.
Liying HuangKey Lab of Biohealth Materials and Chemistry of Wenzhou, College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou, Zhejiang, 325027, People's Republic of China.
Guofeng WangKey Lab of Biohealth Materials and Chemistry of Wenzhou, College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou, Zhejiang, 325027, People's Republic of China.
Nannan LiuKey Lab of Biohealth Materials and Chemistry of Wenzhou, College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou, Zhejiang, 325027, People's Republic of China. liunannan@wzu.edu.cn.

Funding

Natural Science Foundation of Zhejiang Province LY24B050003Open Laboratory Program of Nanjing University SKLACLS2303public industrial technology research projects of Wenzhou G2023042Research Project of Wenzhou 2023G0063
6 · The paper itself

Abstract

The construction of gating system in artificial channels is a cutting-edge research direction in understanding biological process and application sensing. Here, by mimicking the gating system, we report a device that easily synthesized single-glass micropipettes functionalized by three-dimensional (3D) DNA network, which triggers the gating mechanism for the detection of biomolecules. Based on this strategy, the gating mechanism shows that single-glass micropipette assembled 3D DNA network is in the "OFF" state, and after collapsing in the presence of ATP, they are in the "ON" state, at which point they exhibit asymmetric response times. In the "ON" process of the gating mechanism, the ascorbic acid phosphate (AAP) can be encapsulated by a 3D DNA network and released in the presence of adenosine triphosphate (ATP), which initiates a catalyzed cascade reaction under the influence of alkaline phosphatase (ALP). Ultimately, the detection of ALP can be responded to form the fluorescence signal generated by terephthalic acid that has captured hydroxyl radicals, which has a detection range of 0-250 mU/mL and a limit of detection of 50 mU/mL. This work provides a brand-new way and application direction for research of gating mechanism.

Indexed as

Adenosine TriphosphateAlkaline PhosphataseDNAAscorbic AcidBiosensing TechniquesGlassLimit of DetectionAdenosine TriphosphateAlkaline Phosphataseascorbate-2-phosphateAscorbic AcidDNA3D DNA networkATPGating mechanismSingle-glass micropipettes

Identifiers

PMID38795199

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.