Evidence map›Paper›PMID 38793611›Full record

ArticleViruses2024

Droplet Digital RT-PCR (dd RT-PCR) Detection of SARS-CoV-2 in Honey Bees and Honey Collected in Apiaries across the Campania Region.

Andrea Mancusi, Yolande Thérèse Rose Proroga, Paola Maiolino, Raffaele Marrone, Claudia D'Emilio, Santa Girardi, Marica Egidio, Arianna Boni, Teresa Vicenza, Elisabetta Suffredini and 1 more

Abstract read
In one paragraph

Article in Viruses, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Andrea MancusiDepartment of Food Security Coordination, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute No. 2, 80055 Portici, Italy.ORCID 0000-0002-7069-3274
Yolande Thérèse Rose ProrogaDepartment of Food Security Coordination, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute No. 2, 80055 Portici, Italy.ORCID 0000-0002-2748-9286
Paola MaiolinoDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Naples, Italy.ORCID 0000-0002-8863-466X
Raffaele MarroneDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Naples, Italy.ORCID 0000-0002-2888-5846
Claudia D'EmilioDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Naples, Italy.
Santa GirardiDepartment of Food Security Coordination, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute No. 2, 80055 Portici, Italy.
Marica EgidioDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Naples, Italy.
Arianna BoniDepartment of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
Teresa VicenzaDepartment of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
Elisabetta SuffrediniDepartment of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.ORCID 0000-0001-7605-0539
Karen PowerDepartment of Biology, University of Naples Federico II, 80126 Naples, Italy.ORCID 0000-0002-2940-7520

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Coronaviruses (CoVs), a subfamily of Orthocoronavirinae, are viruses that sometimes present a zoonotic character. Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) is responsible for the recent outbreak of COVID-19, which, since its outbreak in 2019, has caused about 774,593,066 confirmed cases and 7,028,881 deaths. Aereosols are the main route of transmission among people; however, viral droplets can contaminate surfaces and fomites as well as particulate matter (PM) in suspensions of natural and human origin. Honey bees are well known bioindicators of the presence of pollutants and PMs in the environment as they can collect a great variety of substances during their foraging activities. The aim of this study was to evaluate the possible role of honey bees as bioindicators of the prevalence SARS-CoV-2. In this regard, 91 samples of honey bees and 6 of honey were collected from different apiaries of Campania region (Southern Italy) in four time periods from September 2020 to June 2022 and were analyzed with Droplet Digital RT-PCR for SARS-CoV-2 target genes Orf1b and N. The screening revealed the presence of SARS-CoV-2 in 12/91 in honey bee samples and in 2/6 honey samples. These results suggest that honey bees could also be used as indicators of outbreaks of airborne pathogens such as SARS-CoV-2.

Indexed as

COVID-19HoneySARS-CoV-2AnimalsBeesHumansItalyReverse Transcriptase Polymerase Chain ReactionRNA, ViralRNA, ViralbioindicatorsDroplet Digital RT-PCRhoney beesSARS-CoV-2

Identifiers

PMID38793611
PMCPMC11126096

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.