Enhancement of Convection and Molecular Transport into Film Stacked Structures by Introduction of Notch Shape for Micro-Immunoassay.

Daiki Arai, Satoshi Ogata, Tetsuhide Shimizu, Ming Yang

Abstract read

In one paragraph

Article in Micromachines, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

From Micro to Marvel: Unleashing the Full Potential of Click Chemistry with Micromachine Integration.Micromachines · 2025
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Daiki AraiDepartment of Mechanical System Engineering, Faculty of System Design, Tokyo Metropolitan University, Tokyo 192-0397, Japan.

Satoshi OgataDepartment of Mechanical System Engineering, Faculty of System Design, Tokyo Metropolitan University, Tokyo 192-0397, Japan.ORCID 0000-0002-5566-9110

Tetsuhide ShimizuDepartment of Mechanical System Engineering, Faculty of System Design, Tokyo Metropolitan University, Tokyo 192-0397, Japan.ORCID 0000-0002-7810-3777

Ming YangDepartment of Mechanical System Engineering, Faculty of System Design, Tokyo Metropolitan University, Tokyo 192-0397, Japan.ORCID 0000-0001-8413-5735

Funding

the Adaptable and Seamless Technology transfer Program through Target-driven R&D (A-STEP) from Japan Science and Technology Agency (JST) Grant , JAPAN JPMJTM20MD

6 · The paper itself

Abstract

A 3D-stack microfluidic device that can be used in combination with 96-well plates for micro-immunoassay was developed by the authors. ELISA for detecting IgA by the 3D-stack can be performed in one-ninth of the time of the conventional method by using only 96-well plates. In this study, a notched-shape film was designed and utilized for the 3D-stack to promote circulation by enhancing and utilizing the axial flow and circumferential flow in order to further reduce the reaction time. A finite element analysis was performed to evaluate the axial flow and circumferential flow while using the 3D-stack in a well and design the optimal shape. The 3D-stack with the notched-shape film was fabricated and utilized for the binding rate test of the antibody and antigen and ELISA. As a result, by promoting circulation using 3D-stack with notched-shape film, the reaction time for each process of ELISA was reduced to 1 min, which is 1/60 for 96 wells at low concentrations.

Indexed as

3D-stackELISAmicrofluidicsnotched-shapeunsteady rotation

Identifiers

PMID38793185

DOI10.3390/mi15050613

PMCPMC11123275

What OpenQuestion holds

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LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.