Nonribosomal Peptide Synthetase Specific Genome Amplification Using Rolling Circle Amplification for Targeted Gene Sequencing.

Yoshiko Okamura, Masahiro Suemitsu, Takato Ishikawa, Hirokazu Takahashi

Abstract read

In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Advances in Virus Detection Techniques Based on Recombinant Polymerase Amplification.Molecules (Basel, Switzerland) · 2024
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Yoshiko OkamuraGraduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima 739-8530, Japan.ORCID 0000-0001-8333-7722

Masahiro SuemitsuGraduate School of Advanced Science of Matter, Hiroshima University, Hiroshima 739-8530, Japan.

Takato IshikawaGraduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima 739-8530, Japan.

Hirokazu TakahashiGraduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima 739-8530, Japan.

Funding

JSPS KAKENHI 15K06583

6 · The paper itself

Abstract

Next-generation sequencing has transformed the acquisition of vast amounts of genomic information, including the rapid identification of target gene sequences in metagenomic databases. However, dominant species can sometimes hinder the detection of rare bacterial species. Therefore, a highly sensitive amplification technique that can selectively amplify bacterial genomes containing target genes of interest was developed in this study. The rolling circle amplification (RCA) method can initiate amplification from a single locus using a specific single primer to amplify a specific whole genome. A mixed cell suspension was prepared using

Indexed as

Genome, BacterialNucleic Acid Amplification TechniquesPeptide SynthasesEscherichia coliHigh-Throughput Nucleotide SequencingMetagenomePseudomonas fluorescensSequence Analysis, DNAnon-ribosomal peptide synthasePeptide Synthasesmetagenomenonribosomal peptide synthetaserolling circle amplificationspecific genome amplificationviable but non-culturable (VBNC) bacteria

Identifiers

PMID38791129

DOI10.3390/ijms25105089

PMCPMC11121399

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.