Evidence map›Paper›PMID 38791067›Full record

ArticleBiomedicines2024

Time- and Region-Specific Selection of Reference Genes in the Rat Brain in the Lithium-Pilocarpine Model of Acquired Temporal Lobe Epilepsy.

Alexander P Schwarz, Maria V Zakharova, Anna A Kovalenko, Alexandra V Dyomina, Olga E Zubareva, Aleksey V Zaitsev

Abstract read
In one paragraph

Article in Biomedicines, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Alexander P SchwarzSechenov Institute of Evolutionary Physiology and Biochemistry of Russian Academy of Sciences, Toreza Prospekt, 44, 194223 Saint Petersburg, Russia.ORCID 0000-0003-2707-1397
Maria V ZakharovaSechenov Institute of Evolutionary Physiology and Biochemistry of Russian Academy of Sciences, Toreza Prospekt, 44, 194223 Saint Petersburg, Russia.
Anna A KovalenkoSechenov Institute of Evolutionary Physiology and Biochemistry of Russian Academy of Sciences, Toreza Prospekt, 44, 194223 Saint Petersburg, Russia.ORCID 0000-0002-3790-2396
Alexandra V DyominaSechenov Institute of Evolutionary Physiology and Biochemistry of Russian Academy of Sciences, Toreza Prospekt, 44, 194223 Saint Petersburg, Russia.ORCID 0000-0001-9751-8874
Olga E ZubarevaSechenov Institute of Evolutionary Physiology and Biochemistry of Russian Academy of Sciences, Toreza Prospekt, 44, 194223 Saint Petersburg, Russia.
Aleksey V ZaitsevSechenov Institute of Evolutionary Physiology and Biochemistry of Russian Academy of Sciences, Toreza Prospekt, 44, 194223 Saint Petersburg, Russia.ORCID 0000-0002-4503-0647

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reverse transcription followed by quantitative polymerase chain reaction (RT-qPCR) is a commonly used tool for gene expression analysis. The selection of stably expressed reference genes is required for accurate normalization. The aim of this study was to identify the optimal reference genes for RT-qPCR normalization in various brain regions of rats at different stages of the lithium-pilocarpine model of acquired epilepsy. We tested the expression stability of nine housekeeping genes commonly used as reference genes in brain research:

Indexed as

acquired epilepsybrainlithium–pilocarpine modelquantitative reverse transcription polymerase chain reactionratreference gene expression stability

Identifiers

PMID38791067
PMCPMC11117783

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.