ArticleNature communications2024
Environment modulates protein heterogeneity through transcriptional and translational stop codon readthrough.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Compositional Characteristics of Macadamia Nuts and Oils from Major Growing Regions in China: A Static Evaluation.Foods (Basel, Switzerland) · 2026Article
- Absolute quantification of fluorescent protein fusions by mass spectrometry.Protein science : a publication of the Protein Society · 2026Article
- Translational control of Toxoplasma gondii differentiation.Bioscience reports · 2026Review
- Protein C-terminal variations impact proteostasis.Nature communications · 2026Article
- Dam-dependent epigenetic memory regulates prophage reintegration in Salmonella.Nucleic acids research · 2025Article
- New dimensions in the molecular genetics of insect chemoreception.Trends in genetics : TIG · 2025Review
- Article
- When "loss-of-function" means proteostasis burden: Thinking again about coding DNA variants.American journal of human genetics · 2025Article
- Evidence of a Protein-Coding Gene Antisense to the UViruses · 2023Article
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Authors and funding
7 authors.
Funding
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Abstract
Stop codon readthrough events give rise to longer proteins, which may alter the protein's function, thereby generating short-lasting phenotypic variability from a single gene. In order to systematically assess the frequency and origin of stop codon readthrough events, we designed a library of reporters. We introduced premature stop codons into mScarlet, which enabled high-throughput quantification of protein synthesis termination errors in E. coli using fluorescent microscopy. We found that under stress conditions, stop codon readthrough may occur at rates as high as 80%, depending on the nucleotide context, suggesting that evolution frequently samples stop codon readthrough events. The analysis of selected reporters by mass spectrometry and RNA-seq showed that not only translation but also transcription errors contribute to stop codon readthrough. The RNA polymerase was more likely to misincorporate a nucleotide at premature stop codons. Proteome-wide detection of stop codon readthrough by mass spectrometry revealed that temperature regulated the expression of cryptic sequences generated by stop codon readthrough in E. coli. Overall, our findings suggest that the environment affects the accuracy of protein production, which increases protein heterogeneity when the organisms need to adapt to new conditions.
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