Evidence map›Paper›PMID 38781967›Full record

ArticleCell2024

Patterning and folding of intestinal villi by active mesenchymal dewetting.

Tyler R Huycke, Teemu J Häkkinen, Hikaru Miyazaki, Vasudha Srivastava, Emilie Barruet, Christopher S McGinnis, Ali Kalantari, Jake Cornwall-Scoones, Dedeepya Vaka, Qin Zhu and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.

0numbers the graph read from it
0cells of the map it votes in
50citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

50 citing papers in PubMed.

  1. Regional organization of nutrient absorption across the small intestine.Nature reviews. Gastroenterology & hepatology · 2026
    Review
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  3. Article
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  5. What is active wetting?The European physical journal. E, Soft matter · 2026
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  10. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

19 authors.

Tyler R HuyckeDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA; Program in Craniofacial Biology and Department of Orofacial Sciences, University of California, San Francisco, San Francisco, CA, USA.
Teemu J HäkkinenDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA; Program in Craniofacial Biology and Department of Orofacial Sciences, University of California, San Francisco, San Francisco, CA, USA.
Hikaru MiyazakiDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA; Program in Craniofacial Biology and Department of Orofacial Sciences, University of California, San Francisco, San Francisco, CA, USA.
Vasudha SrivastavaDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA.
Emilie BarruetDepartment of Pediatrics, Cedars-Sinai Guerin Children's, Los Angeles, CA, USA.
Christopher S McGinnisDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA.
Ali KalantariDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA; Program in Craniofacial Biology and Department of Orofacial Sciences, University of California, San Francisco, San Francisco, CA, USA.
Jake Cornwall-ScoonesDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Dedeepya VakaDepartment of Pediatrics, Cedars-Sinai Guerin Children's, Los Angeles, CA, USA.
Qin ZhuDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA.
Hyunil JoDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA.
Roger OriaCenter for Bioengineering and Tissue Regeneration, Department of Surgery, University of California, San Francisco, San Francisco, CA 94143, USA; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA 94143, USA; Comprehensive Cancer Center, Helen Diller Family Cancer Research Center, University of California, San Francisco, San Francisco, CA 94143, USA; Department of Bioengineering and Therapeutic Sciences, Department of Radiation Oncology, University of California, San Francisco, San Francisco, CA 94143, USA.
Valerie M WeaverCenter for Bioengineering and Tissue Regeneration, Department of Surgery, University of California, San Francisco, San Francisco, CA 94143, USA; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA 94143, USA; Comprehensive Cancer Center, Helen Diller Family Cancer Research Center, University of California, San Francisco, San Francisco, CA 94143, USA; Department of Bioengineering and Therapeutic Sciences, Department of Radiation Oncology, University of California, San Francisco, San Francisco, CA 94143, USA.
William F DeGradoDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA.
Matt ThomsonDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Krishna GarikipatiDepartments of Mechanical Engineering, and Mathematics, University of Michigan, Ann Arbor, MI, USA.
Dario BoffelliDepartment of Pediatrics, Cedars-Sinai Guerin Children's, Los Angeles, CA, USA.
Ophir D KleinProgram in Craniofacial Biology and Department of Orofacial Sciences, University of California, San Francisco, San Francisco, CA, USA; Department of Pediatrics, Cedars-Sinai Guerin Children's, Los Angeles, CA, USA. Electronic address: ophir.klein@cshs.org.
Zev J GartnerDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA; Chan Zuckerberg Biohub, San Francisco, CA, USA. Electronic address: zev.gartner@ucsf.edu.

Funding

Harnessing natural stem cell-based strategies for mammalian dental renewalR35DE026602 · NIDCR · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI KLEIN, OPHIR D · 2016 to 2023
$8.0M
The roles of regional specialization, mechanical forces and epigenetic memory after perturbation and injury of the intestinal stem cell microenvironmentU01DK103147 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI KLEIN, OPHIR D · 2014 to 2023
$5.4M
The physical and molecular mechanisms of intestinal villus morphogenesis and repairR01DK126376 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Zev Jordan Gartner · 2020 to 2026
$4.2M
Understanding breast cancer progression as a defect in the mechanics of tissue self-organizationU01CA244109 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GARTNER, ZEV JORDAN, GOGA, ANDREI · 2020 to 2024
$3.0M
MULTIseq: multiplexing massively parallel single cell transcriptional analysis across time, space, and conditionsR01GM135462 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GARTNER, ZEV JORDAN · 2019 to 2022
$1.3M
Universal Sample Multiplexing for Single Cell AnalysisR33CA247744 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GARTNER, ZEV JORDAN · 2021 to 2023
$1.2M
Mechanochemical mechanisms of intestinal villus development and regenerationF32DK128949 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI HUYCKE, TYLER · 2021 to 2023
$205k
NCI NIH HHS R33 CA247744NCI NIH HHS U01 CA244109NIDCR NIH HHS R35 DE026602NIDDK NIH HHS F32 DK128949NIDDK NIH HHS R01 DK126376NIDDK NIH HHS U01 DK103147NIGMS NIH HHS R01 GM135462
6 · The paper itself

Abstract

Tissue folds are structural motifs critical to organ function. In the intestine, bending of a flat epithelium into a periodic pattern of folds gives rise to villi, finger-like protrusions that enable nutrient absorption. However, the molecular and mechanical processes driving villus morphogenesis remain unclear. Here, we identify an active mechanical mechanism that simultaneously patterns and folds the intestinal epithelium to initiate villus formation. At the cellular level, we find that PDGFRA+ subepithelial mesenchymal cells generate myosin II-dependent forces sufficient to produce patterned curvature in neighboring tissue interfaces. This symmetry-breaking process requires altered cell and extracellular matrix interactions that are enabled by matrix metalloproteinase-mediated tissue fluidization. Computational models, together with in vitro and in vivo experiments, revealed that these cellular features manifest at the tissue level as differences in interfacial tensions that promote mesenchymal aggregation and interface bending through a process analogous to the active dewetting of a thin liquid film.

Indexed as

Extracellular MatrixIntestinal MucosaAnimalsMatrix MetalloproteinasesMesenchymal Stem CellsMesodermMiceMorphogenesisMyosin Type IIReceptor, Platelet-Derived Growth Factor alphaMatrix MetalloproteinasesMyosin Type IIReceptor, Platelet-Derived Growth Factor alphaactive fluidsbiophysicsCahn-Hilliardcell adhesiondevelopmentextracellular matrixmorphogenesispatterningphase separationself-organization

Identifiers

PMID38781967
PMCPMC11166531

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.