ArticleiScience2024
KLF4 promotes milk fat synthesis by regulating the PI3K-AKT-mTOR pathway and targeting FASN activation in bovine mammary epithelial cells.
Article in iScience, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Functional analysis and identification of miRNAs associated with lipid metabolism from milk-derived exosomes.Journal of animal science and biotechnology · 2026Article
- Mining of candidate genes related to prolificacy in Jining grey goats using transcriptomics.BMC genomics · 2025Article
- DeepBovC2H2-ZF: deep learning-guided prediction and molecular dynamics validation of C2H2 zinc finger transcription factors in Bovidae.Journal, genetic engineering & biotechnology · 2025Article
- MicroRNA-30a-3p Influences Milk Fat Metabolism by TargetingAnimals : an open access journal from MDPI · 2025Article
- Integrated gene co-expression network analysis and experimental validation identified integrin beta 1 as a candidate gene affecting milk production in dairy cattle.Journal of animal science · 2025Article
- Zearalenone triggers programmed cell death and impairs milk fat synthesis via the AKT-mTOR-PPARγ-ACSL4 pathway in bovine mammary epithelial cells.Journal of animal science · 2024Article
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Authors and funding
7 authors.
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Abstract
Milk fat is an important indicator for evaluating the quality of cow's milk. In this study, we used bovine mammary epithelial cells (BMECs) to investigate the role and molecular mechanism of KLF4 in the regulation of milk fat synthesis. The results showed that KLF4 was more highly expressed in mammary tissues of high-fat cows compared with low-fat cows. KLF4 positively regulated the expression of genes related to milk fat synthesis in BMECs, increasing intracellular triglycerides content, and KLF4 promoted milk fat synthesis by activating the PI3K-AKT-mTOR signaling pathway. Furthermore, the results of animal experiments also confirmed that knockdown of KLF4 inhibited milk fat synthesis. In addition, yeast one-hybrid assays and dual-luciferase reporter gene assays confirmed that KLF4 directly targets and binds to the fatty acid synthase (FASN) promoter region to promote FASN transcription. These results demonstrate that KLF4 is a key transcription factor for milk fat synthesis in BMECs.
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