Evidence map›Paper›PMID 38777961›Full record

ArticleJournal of assisted reproduction and genetics2024

Supplementation with autologous adipose stem cell-derived mitochondria can be a safe and promising strategy for improving oocyte quality.

Sanath Udayanga Kankanam Gamage, Shu Hashimoto, Yuki Miyamoto, Tatsuya Nakano, Masaya Yamanaka, Hideki Kitaji, Yuki Takada, Hiroshi Matsumoto, Akiko Koike, Manabu Satoh and 3 more

Registry-linked trialAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT07066267 (Clinical Application of Autologous Adipose Stem Mitochondria Transplantation to Oocytes for Improving Embryo Quality in Patients With Recurrent Pregnancy Failure), which is not on this map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT07066267 narecruitingnot on this mapstarted 2025, after this paper: background citation

Clinical Application of Autologous Adipose Stem Mitochondria Transplantation to Oocytes for Improving Embryo Quality in Patients With Recurrent Pregnancy Failure

TypeinterventionalSponsorSunkaky Medical CooperationRan2025 to 2026Enrolled20ConditionsFemale Infertility, Oocyte Competence, Mitochondria, Recurent Implantation FailuresArmsExperimental
3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Sanath Udayanga Kankanam GamageHORAC Grand Front Osaka Clinic, Osaka, Japan. udayanga646@ivfjapan.com.ORCID http://orcid.org/0000-0002-2198-1069
Shu HashimotoReproductive Science Institute, Graduate School of Medicine, Osaka Metropolitan University, Osaka, Japan.ORCID http://orcid.org/0000-0001-9083-1403
Yuki MiyamotoHORAC Grand Front Osaka Clinic, Osaka, Japan.
Tatsuya NakanoIVF Namba Clinic, Osaka, Japan.
Masaya YamanakaIVF Namba Clinic, Osaka, Japan.
Hideki KitajiReproductive Science Institute, Graduate School of Medicine, Osaka Metropolitan University, Osaka, Japan.
Yuki TakadaReproductive Science Institute, Graduate School of Medicine, Osaka Metropolitan University, Osaka, Japan.ORCID http://orcid.org/0000-0003-4710-3448
Hiroshi MatsumotoIVF Osaka Clinic, Osaka, Japan.
Akiko KoikeHORAC Grand Front Osaka Clinic, Osaka, Japan.
Manabu SatohIVF Namba Clinic, Osaka, Japan.
Masako IchishiDepartment of Oncologic Pathology, Graduate School of Medicine, Mie University, Mie, Japan.
Masatoshi WatanabeDepartment of Oncologic Pathology, Graduate School of Medicine, Mie University, Mie, Japan.ORCID http://orcid.org/0000-0002-9423-0901
Yoshiharu MorimotoHORAC Grand Front Osaka Clinic, Osaka, Japan.ORCID http://orcid.org/0000-0003-2834-2726

Funding

Japan Society for the Promotion of Science 20K09674
6 · The paper itself

Abstract

purposeIn our previous study, we confirmed that the supplementation of vitrified-warmed murine oocytes with autologous adipose stem cell (ASC)-derived mitochondria during intracytoplasmic sperm injection enhances post-fertilization developmental competence in mice. To ensure the safety of this technology, we conducted a thorough study in mice to investigate the potential presence of specific malformations in offspring developed from this approach.

methodsA transgenerational comparative analysis was conducted on founder mice from embryos that developed after mitochondrial supplementation, and two subsequent generations. Reproductive performance, body growth rate, histopathological parameters, hematological parameters, daily activity patterns, and daily body temperature changes in male and female mice across these three generations were assessed in comparison to wild-type mice of the same age.

resultsBoth male and female animals in all three generations showed comparable reproductive performance to the control group. Additionally, body growth rate by the age of 8 weeks were found to be comparable to controls across all three generations. Notably, no significant histopathological abnormalities were detected in vital organs, including the brain, heart, liver, kidneys, lungs, ovaries, and testes, in any individuals from the studied cohorts. The blood parameters were consistent with the control data. The continuous monitoring of activity and body temperature changes (both day and night) over a 1-week period revealed a pattern closely resembling that observed in the control animals.

conclusionInjection of ASC-mitochondria into oocytes may be a promising technique to support developmental potential without causing adverse epigenetic events in the offspring in mice. However, before considering clinical application, additional safety screening using larger animals or non-human primates is essential.

Indexed as

MitochondriaOocytesSperm Injections, IntracytoplasmicAdipose TissueAnimalsCryopreservationFemaleHumansMaleMiceStem CellsAdipose stem cellMitochondrial supplementationOocyte quality enhancementTransgenerational safety

Identifiers

PMID38777961
PMCPMC11339003

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Registered trials

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