ArticleMed (New York, N.Y.)2024
Cellular dynamics in pig-to-human kidney xenotransplantation.
Article in Med (New York, N.Y.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Vascularized composite xenotransplantation: Where we have been and where we could go?Cell transplantationPooled it
- Hemoglobin-based oxygen carrier: what we have learned and where we are heading?Protein & cell · 2026Article
- Article
- Longitudinal multiomics profiling of extracorporeal cross-circulation with pig liver xenografts in human decedents.Nature medicine · 2026Article
- VCAM-1-targeting peptide assemblies protect vascular endothelium and prolong cardiac xenograft survival.Science advances · 2026Article
- Review
- Present Advances and Emerging Challenges in Kidney Xenotransplantation.Journal of clinical medicine · 2026Review
- Article
- Orthotopic Porcine Liver and Kidney Xenotransplantation as a Step Toward Multi-Organ Replacement.International journal of biological sciences · 2026Article
- T cell-mediated immune responses in xenotransplantation: mechanisms and therapeutic strategies.Frontiers in immunology · 2026Review
- Review
- Immune profiling in a living human recipient of a gene-edited pig kidney.Nature medicine · 2026Article
- Specific pathogen free ten gene-edited donor pigs for xenotransplantation.Protein & cell · 2025Article
- Coordinated circulating and tissue-based T cell responses precede xenograft rejection.bioRxiv : the preprint server for biology · 2025Article
- Generation of exogenous kidneys via CRISPR/Cas9 mediated blastocyst complementation targeting Osr1 gene in mice.Scientific reports · 2025Article
- Xenophagocytosis blockade enhances interspecies chimerism.bioRxiv : the preprint server for biology · 2025Article
- Plasma exchange and intravenous immunoglobulin prolonged the survival of a porcine kidney xenograft in a sensitized, brain-dead human recipient.Chinese medical journal · 2025Article
- Pluripotent stem cell-derived cardiomyocyte transplantation: marching from bench to bedside.Science China. Life sciences · 2025Review
- Immune cell landscape in a human decedent receiving a pig liver xenograft.Nature medicine · 2025Article
- Scientific advances in the assessment, modification, and generation of transplantable organs for patients with end-stage organ diseases.Lancet (London, England) · 2025Review
Corrections and comments
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Authors and funding
21 authors.
Funding
Abstract
backgroundXenotransplantation of genetically engineered porcine organs has the potential to address the challenge of organ donor shortage. Two cases of porcine-to-human kidney xenotransplantation were performed, yet the physiological effects on the xenografts and the recipients' immune responses remain largely uncharacterized.
methodsWe performed single-cell RNA sequencing (scRNA-seq) and longitudinal RNA-seq analyses of the porcine kidneys to dissect xenotransplantation-associated cellular dynamics and xenograft-recipient interactions. We additionally performed longitudinal scRNA-seq of the peripheral blood mononuclear cells (PBMCs) to detect recipient immune responses across time.
findingsAlthough no hyperacute rejection signals were detected, scRNA-seq analyses of the xenografts found evidence of endothelial cell and immune response activation, indicating early signs of antibody-mediated rejection. Tracing the cells' species origin, we found human immune cell infiltration in both xenografts. Human transcripts in the longitudinal bulk RNA-seq revealed that human immune cell infiltration and the activation of interferon-gamma-induced chemokine expression occurred by 12 and 48 h post-xenotransplantation, respectively. Concordantly, longitudinal scRNA-seq of PBMCs also revealed two phases of the recipients' immune responses at 12 and 48-53 h. Lastly, we observed global expression signatures of xenotransplantation-associated kidney tissue damage in the xenografts. Surprisingly, we detected a rapid increase of proliferative cells in both xenografts, indicating the activation of the porcine tissue repair program.
conclusionsLongitudinal and single-cell transcriptomic analyses of porcine kidneys and the recipient's PBMCs revealed time-resolved cellular dynamics of xenograft-recipient interactions during xenotransplantation. These cues can be leveraged for designing gene edits and immunosuppression regimens to optimize xenotransplantation outcomes.
fundingThis work was supported by NIH RM1HG009491 and DP5OD033430.
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