Evidence map›Paper›PMID 38775491›Full record

ArticleApplied and environmental microbiology2024

Mobile-CRISPRi as a powerful tool for modulating

Logan J Geyman, Madeline P Tanner, Natalia Rosario-Meléndez, Jason M Peters, Mark J Mandel, Julia C van Kessel

Abstract read
In one paragraph

Article in Applied and environmental microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Anti-QS Strategies AgainstMicroorganisms · 2025
    Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Mobile-CRISPRi protocol optimization forMicrobiology resource announcements · 2024
    Article
  7. Lighting the way: how theJournal of bacteriology · 2024
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Logan J GeymanDepartment of Biology, Indiana University, Bloomington, Indiana, USA.ORCID 0000-0001-6697-6725
Madeline P TannerDepartment of Microbiology, School of Medicine, University of Washington, Seattle, Washington, USA.
Natalia Rosario-MeléndezDepartment of Medical Microbiology & Immunology, University of Wisconsin-Madison, Madison, Wisconsin, USA.ORCID 0000-0003-1665-4167
Jason M PetersMicrobiology Doctoral Training Program, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Mark J MandelDepartment of Medical Microbiology & Immunology, University of Wisconsin-Madison, Madison, Wisconsin, USA.ORCID 0000-0001-6506-6711
Julia C van KesselDepartment of Biology, Indiana University, Bloomington, Indiana, USA.ORCID 0000-0002-1612-2403

Funding

Biotechnology Training ProgramT32GM135066 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI SCOTT M. COYLE, BRIAN G FOX · 2020 to 2026
$7.0M
Quorum sensing regulation of bacterial developmentR35GM124698 · NIGMS · TRUSTEES OF INDIANA UNIVERSITY · PI Julia C. van Kessel · 2017 to 2026
$4.1M
Genetic analysis of beneficial bacterial colonizationR35GM148385 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI Mark J Mandel · 2023 to 2026
$1.8M
Cross-species Analysis of Bacterial Gene NetworksR35GM150487 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI Jason M. Peters · 2023 to 2026
$1.5M
HHS | National Institutes of Health (NIH) R35GM124698HHS | National Institutes of Health (NIH) R35GM148385HHS | National Institutes of Health (NIH) R35GM150487HHS | National Institutes of Health (NIH) T32GM135066National Science Foundation (NSF) GRFPNIGMS NIH HHS R35 GM124698NIGMS NIH HHS R35 GM148385NIGMS NIH HHS R35 GM150487NIGMS NIH HHS T32 GM135066
6 · The paper itself

Abstract

CRISPRi (Clustered Regularly Interspaced Palindromic Repeats interference) is a gene knockdown method that uses a deactivated Cas9 protein (dCas9) that binds a specific gene target locus dictated by an encoded guide RNA (sgRNA) to block transcription. Mobile-CRISPRi is a suite of modular vectors that enable CRISPRi knockdowns in diverse bacteria by integrating IPTG-inducible

Indexed as

VibrioVibrio vulnificusAliivibrio fischeriBacterial ProteinsClustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Cas SystemsGene Expression Regulation, BacterialGene Knockdown TechniquesVibrio choleraeVibrio parahaemolyticusBacterial ProteinsCRISPRCRISPRigene expressionVibrioVibrio campbelliiVibrio choleraeVibrio fischeriVibrio parahaemolyticusVibrio vulnificus

Identifiers

PMID38775491
PMCPMC11218623

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.