Evidence map›Paper›PMID 38775133›Full record

ArticleeLife2024

Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brain.

Ya-Hui Lin, Li-Wen Wang, Yen-Hui Chen, Yi-Chieh Chan, Shang-Hsiu Hu, Sheng-Yan Wu, Chi-Shiun Chiang, Guan-Jie Huang, Shang-Da Yang, Shi-Wei Chu and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in eLife, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Ya-Hui LinDepartment of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, Taiwan.ORCID https://orcid.org/0000-0001-9130-5149
Li-Wen WangDepartment of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, Taiwan.
Yen-Hui ChenInstitute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
Yi-Chieh ChanDepartment of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, Taiwan.
Shang-Hsiu HuDepartment of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, Taiwan.
Sheng-Yan WuDepartment of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, Taiwan.
Chi-Shiun ChiangDepartment of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, Taiwan.ORCID https://orcid.org/0000-0002-2581-4129
Guan-Jie HuangDepartment of Physics, National Taiwan University, Taipei, Taiwan.
Shang-Da YangInstitute of Photonics Technologies, National Tsing Hua University, Hsinchu, Taiwan.
Shi-Wei ChuDepartment of Physics, National Taiwan University, Taipei, Taiwan.ORCID https://orcid.org/0000-0001-7728-4329
Kuo-Chuan WangDepartment of Neurosurgery, National Taiwan University Hospital, Taipei, Taiwan.
Chin-Hsien LinDepartment of Neurosurgery, National Taiwan University Hospital, Taipei, Taiwan.
Pei-Hsin HuangDepartment of Pathology, National Taiwan University Hospital, Taipei, Taiwan.
Hwai-Jong ChengInstitute of Molecular Biology, Academia Sinica, Taipei, Taiwan.
Bi-Chang ChenResearch Center for Applied Sciences, Academia Sinica, Taipei, Taiwan.
Li-An ChuDepartment of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, Taiwan.ORCID https://orcid.org/0000-0002-4092-3024

Funding

National Science and Technology Council MOST 110-2321-B-010-006National Science and Technology Council NSTC 07-2314-B-007-003-MY3National Science and Technology Council NSTC 110-2321-B-010-006National Science and Technology Council NSTC-111-2321-B-002-016National Science and Technology Council NSTC 111-2636-B-007-007
6 · The paper itself

Abstract

Tissue-clearing and labeling techniques have revolutionized brain-wide imaging and analysis, yet their application to clinical formalin-fixed paraffin-embedded (FFPE) blocks remains challenging. We introduce HIF-Clear, a novel method for efficiently clearing and labeling centimeter-thick FFPE specimens using elevated temperature and concentrated detergents. HIF-Clear with multi-round immunolabeling reveals neuron circuitry regulating multiple neurotransmitter systems in a whole FFPE mouse brain and is able to be used as the evaluation of disease treatment efficiency. HIF-Clear also supports expansion microscopy and can be performed on a non-sectioned 15-year-old FFPE specimen, as well as a 3-month formalin-fixed mouse brain. Thus, HIF-Clear represents a feasible approach for researching archived FFPE specimens for future neuroscientific and 3D neuropathological analyses.

Indexed as

BrainFormaldehydeNeuronsParaffin EmbeddingTissue FixationAnimalsFixativesMiceFixativesFormaldehydearchived tissuesFFPEhumanmousemultiplexed stainingneurosciencetissue clearingwhole brain analysiswhole mouse brain imaging

Identifiers

PMID38775133
PMCPMC11111220

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.