Evidence map›Paper›PMID 38773624›Full record

ArticleMolecular brain2024

A method for selective and efficient isolation of gray matter astrocytes from the spinal cord of adult mice.

Ryoma Iwasaki, Yuta Kohro, Makoto Tsuda

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular brain, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Ryoma Iwasaki *Department of Molecular and System Pharmacology, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
Yuta Kohro *Department of Molecular and System Pharmacology, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.ORCID 0000-0002-3128-7388
Makoto TsudaDepartment of Molecular and System Pharmacology, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan. tsuda@phar.kyushu-u.ac.jp.ORCID 0000-0003-0585-9570

Funding

Core Research for Evolutional Science and Technology JP24gm1510013hJapan Agency for Medical Research and Development JP24ama121031Japan Society for the Promotion of Science JP19H05658Japan Society for the Promotion of Science JP20H05900Uehara Memorial Foundation Uehara Memorial Foundation
6 · The paper itself

Abstract

A growing body of evidence indicates intra- and inter-regional heterogeneity of astrocytes in the brain. However, because of a lack of an efficient method for isolating astrocytes from the spinal cord, little is known about how much spinal cord astrocytes are heterogeneous in adult mice. In this study, we developed a new method for isolating spinal astrocytes from adult mice using a cold-active protease from Bacillus licheniformis with an astrocyte cell surface antigen-2 (ACSA-2) antibody. Using fluorescence-activated cell sorting, isolated spinal ACSA-2

Indexed as

AstrocytesCell SeparationGray MatterSpinal CordAgingAnimalsMaleMiceMice, Inbred C57BLRNA, MessengerRNA, MessengerACSA-2 antibodyAstrocytesCold-active proteaseDRAQ5Fluorescence-activated cell sortingGray matterSpinal cord

Identifiers

PMID38773624
PMCPMC11106874

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.