Evidence map›Paper›PMID 38773374›Full record

ArticleBMC genomics2024

Protein expression patterns and metal metabolites in a protogynous hermaphrodite fish, the ricefield eel (Monopterus albus).

Zhi He, Feng Xiao, Deying Yang, Faqiang Deng, Wenxiang Ding, Zhide He, Siqi Wang, Qiqi Chen, Haochen Wang, Mingqiang Chen and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Zhi He *College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Feng Xiao *College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Deying Yang *College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Faqiang DengCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Wenxiang DingCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Zhide HeFish Resources and Environment in Upper Reaches of the Yangtze River Observation and Research Station of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Siqi WangCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Qiqi ChenCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Haochen WangCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Mingqiang ChenCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Kuo GaoCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Jinxing XiongCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Ziting TangCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Mingwang ZhangCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Taiming YanCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China. yantaiming@sicau.edu.cn.

Funding

National Natural Science Foundation of China 31972777
6 · The paper itself

Abstract

backgroundThe ricefield eel Monopterus albus undergoes a natural sex change from female to male during its life cycle, and previous studies have shown the potential mechanisms of this transition at the transcriptional and protein levels. However, the changes in protein levels have not been fully explored, especially in the intersexual stage.

resultsIn the present study, the protein expression patterns in the gonadal tissues from five different periods, the ovary (OV), early intersexual stage gonad (IE), middle intersexual stage gonad (IM), late intersexual stage gonad (IL), and testis (TE), were determined by untargeted proteomics sequencing. A total of 5125 proteins and 394 differentially expressed proteins (DEPs) were detected in the gonadal tissues. Of the 394 DEPs, there were 136 between the OV and IE groups, 20 between the IM and IE groups, 179 between the IL and IM groups, and 59 between the TE and IL groups. Three candidate proteins, insulin-like growth factor 2 mRNA-binding protein 3 isoform X1 (Igf2bp3), triosephosphate isomerase (Tpi), and Cu-Zn superoxide dismutase isoform X1 [(Cu-Zn) Sod1], were validated by western blotting to verify the reliability of the data. Furthermore, metal metabolite-related proteins were enriched in the IL vs. IM groups and TE vs. IL groups, which had close relationships with sex change, including Cu

conclusionsIn summary, we analyzed the protein profiles of ricefield eel gonadal tissues in five sexual stages (OV, IE, IM, IL, and TE) and verified the plausibility of the data. After preforming the functional enrichment of metal metabolite-related DEPs, we detected the contents of the metal metabolites Zn

Indexed as

MetalsAnimalsEelsFemaleFish ProteinsGene Expression ProfilingHermaphroditic OrganismsMaleProteomicsSmegmamorphaTestisFish ProteinsMetalsMetal metabolitesMonopterus albusProteomic analysisSex change

Identifiers

PMID38773374
PMCPMC11106920

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.