ArticleNature communications2024
Fluorescent fatty acid conjugates for live cell imaging of peroxisomes.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Discerning protein pools by selective staining with self-labeling tags.FEBS letters · 2026Review
- Organelle-specific fluorescent probes for live imaging at the nanoscale.Science China. Life sciences · 2026Review
- Peroxisomal import is circadian in glia and regulates sleep and lipid metabolism.PLoS biology · 2026Article
- A mitochondria-driven quality control mechanism for peroxisomal membrane proteins.Nature communications · 2026Article
- Visualisation of peroxisomes: a journey through seven decades.Histochemistry and cell biology · 2026Review
- Article
- Enzyme-Activated Dual-Locked Probes for Detecting Nitroreductase and Carboxylesterase.Chemical & biomedical imaging · 2026Article
- Machine Learning-Assisted Quantification of Organelle Abundance.Bio-protocol · 2026Article
- Real-Time Tracking of Intracellular Prenyl Phosphate Pools in the Marine DiatomACS synthetic biology · 2026Article
- VAPB is a negative regulator of STING-mediated innate immune signaling.Science advances · 2025Article
- Protein Kinase C promotes peroxisome biogenesis and peroxisome-endoplasmic reticulum interaction.The Journal of cell biology · 2025Article
- Near-infrared fatty acid molecular probe for image-guided surgery of glioblastoma.Npj imaging · 2025Article
- Lipid Metabolism and Immune Function: Chemical Tools for Insights into T-Cell Biology.Chembiochem : a European journal of chemical biology · 2025Review
- Tools to Dissect Lipid Droplet Regulation, Players, and Mechanisms.ACS chemical biology · 2025Review
- Modelling Peroxisomal Disorders in Zebrafish.Cells · 2025Review
- Seventy years of peroxisome research: current advances and future perspectives.Histochemistry and cell biology · 2025Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Peroxisomes are eukaryotic organelles that are essential for multiple metabolic pathways, including fatty acid oxidation, degradation of amino acids, and biosynthesis of ether lipids. Consequently, peroxisome dysfunction leads to pediatric-onset neurodegenerative conditions, including Peroxisome Biogenesis Disorders (PBD). Due to the dynamic, tissue-specific, and context-dependent nature of their biogenesis and function, live cell imaging of peroxisomes is essential for studying peroxisome regulation, as well as for the diagnosis of PBD-linked abnormalities. However, the peroxisomal imaging toolkit is lacking in many respects, with no reporters for substrate import, nor cell-permeable probes that could stain dysfunctional peroxisomes. Here we report that the BODIPY-C12 fluorescent fatty acid probe stains functional and dysfunctional peroxisomes in live mammalian cells. We then go on to improve BODIPY-C12, generating peroxisome-specific reagents, PeroxiSPY650 and PeroxiSPY555. These probes combine high peroxisome specificity, bright fluorescence in the red and far-red spectrum, and fast non-cytotoxic staining, making them ideal tools for live cell, whole organism, or tissue imaging of peroxisomes. Finally, we demonstrate that PeroxiSPY enables diagnosis of peroxisome abnormalities in the PBD CRISPR/Cas9 cell models and patient-derived cell lines.
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Registered trials
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