Evidence map›Paper›PMID 38773043›Full record

ArticleBiochemical genetics2025

Studying Human Pathogenic Cryptococcus Gattii Lineages by Utilizing Simple Sequence Repeats to Create Diagnostic Markers and Analyzing Diversity.

Mohd Adnan Kausar, Jitendra Narayan, Nishtha Mishra, Yusuf Akhter, Rajeev Singh, Amany Mohammed Khalifa, Amel Bakri Mohammed El-Hag, Ruba Mustafa Elsaid Ahmed, Neetu Tyagi, Sahil Mahfooz

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Article in Biochemical genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Mohd Adnan KausarDepartment of Biochemistry, College of Medicine, University of Ha'il, Hail, 2440, Saudi Arabia. ma.kausar@uoh.edu.sa.
Jitendra NarayanCSIR- Institute of Genomics and Integrative Biology, Mall Road, New Delhi, 110007, India.
Nishtha MishraDepartment of Chemistry, Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur, 273009, India.
Yusuf AkhterDepartment of Biotechnology, Babasaheb Bhimrao Ambedkar University, Lucknow, 226025, India.
Rajeev SinghDepartment of Environmental Science, Jamia Millia Islamia Central University, New Delhi, 110025, India.
Amany Mohammed KhalifaDepartment of Pathology, College of Medicine, University of Ha'il, Hail, 2440, Saudi Arabia.
Amel Bakri Mohammed El-HagDepartment of Pathology, College of Medicine, University of Ha'il, Hail, 2440, Saudi Arabia.
Ruba Mustafa Elsaid AhmedDepartment of Pathology, College of Medicine, University of Ha'il, Hail, 2440, Saudi Arabia.
Neetu TyagiBone Biology Laboratory, Department of Physiology, University of Louisville, Louisville, USA.
Sahil MahfoozDepartment of Industrial Microbiology, Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur, 273009, India. sahil77786@gmail.com.

Funding

Scientific Research Deanship at the University of Ha'il - Saudi Arabia RG-21 152
6 · The paper itself

Abstract

In this study, we compared the occurrence, relative abundance (RA), and density (RD) of simple sequence repeats (SSRs) among the lineages of human pathogenic Cryptococcus gattii using an in-silico approach to gain a deeper understanding of the structure and evolution of their genomes. C. gattii isolate MF34 showed the highest RA and RD of SSRs in both the genomic and transcriptomic sequences, followed by isolate WM276. In both the genomic (50%) and transcriptomic (65%) sequences, trinucleotide SSRs were the most common SSR class. A motif conservation study found that the isolates had stronger conservation (56.1%) of motifs, with isolate IND107 having the most (5.7%) unique motifs. We discovered the presence of SSRs in genes that are directly or indirectly associated with disease using gene enrichment analysis. Isolate-specific unique motifs identified in this study could be utilized as molecular probes for isolate identification. To improve genetic resources among C. gattii isolates, 6499 primers were developed. These genomic resources developed in this study could help with diversity analysis and the development of isolate-specific markers.

Indexed as

CryptococcosisCryptococcus gattiiGenetic VariationMicrosatellite RepeatsGenetic MarkersGenome, FungalHumansGenetic MarkersDiversityGene enrichmentGenomic resourcesHuman pathogensMotif conservationSimple sequence repeats

Identifiers

PMID38773043

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.