Evidence map›Paper›PMID 38772984›Full record

ArticleImmunologic research2024

The role of tumor-associated macrophages in the radioresistance of esophageal cancer cells via regulation of the VEGF-mediated angiogenic pathway.

Fei Sun, Yingying Lian, Mengyun Zhou, Judong Luo, Lijun Hu, Jianlin Wang, Zhiqiang Sun, Jingping Yu

Abstract read
PubMed Publisher
In one paragraph

Article in Immunologic research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Fei Sun *Department of Radiotherapy, The Affiliated Changzhou Second People's Hospital of Nanjing Medical University, No. 29, Xinglong Lane, Tianning District, Changzhou, 213003, Jiangsu Province, China.
Yingying Lian *Department of Radiotherapy, The Affiliated Changzhou Second People's Hospital of Nanjing Medical University, No. 29, Xinglong Lane, Tianning District, Changzhou, 213003, Jiangsu Province, China.
Mengyun ZhouDepartment of Radiotherapy, The Affiliated Changzhou Second People's Hospital of Nanjing Medical University, No. 29, Xinglong Lane, Tianning District, Changzhou, 213003, Jiangsu Province, China.
Judong LuoDepartment of Radiotherapy, The Affiliated Changzhou Second People's Hospital of Nanjing Medical University, No. 29, Xinglong Lane, Tianning District, Changzhou, 213003, Jiangsu Province, China.
Lijun HuDepartment of Radiotherapy, The Affiliated Changzhou Second People's Hospital of Nanjing Medical University, No. 29, Xinglong Lane, Tianning District, Changzhou, 213003, Jiangsu Province, China.
Jianlin WangDepartment of Radiotherapy, The Affiliated Changzhou Second People's Hospital of Nanjing Medical University, No. 29, Xinglong Lane, Tianning District, Changzhou, 213003, Jiangsu Province, China.
Zhiqiang SunDepartment of Radiotherapy, The Affiliated Changzhou Second People's Hospital of Nanjing Medical University, No. 29, Xinglong Lane, Tianning District, Changzhou, 213003, Jiangsu Province, China.
Jingping YuChangzhou Maternal and Child Health Care Hospital, Changzhou Medical Center, Nanjing Medical University, Changzhou, 213003, Jiangsu Province, China. yujingping@njmu.edu.cn.

Funding

Changzhou Science and techonology Program CZ20210030Major science and technology project of Changzhou Health Commission ZD202017Major science and technology project of Changzhou Health Commission ZD202313the National Natural Science Foundation of China 82073339Young Talent Development Plan of Changzhou Health Commission CZQM2020074Youth Program of Changzhou Second People's Hospital 2020K003
6 · The paper itself

Abstract

Tumor-associated macrophages (TAMs) are known to promote tumor growth, invasion, metastasis, and protumor angiogenesis, but the role of TAMs in evading radiotherapy in esophagus cancer remains unclear. In this study, we first induced TAMs from human monocytes (THP-1) and identified using immunofluorescence and Western blotting assays. We then co-cultured them with human esophageal cancer cell lines. CCK-8, colony formation, Transwell, scratch test, and TUNEL assays showed that TAMs could promote proliferation, survival rate, invasion, migration, and radioresistance and could inhibit apoptosis of the esophageal squamous carcinoma cell lines KYSE-150 and TE-1 before and after radiotherapy both in vivo and in vitro. Using LV-VEGFA-RNAi lentiviral vectors, we also found that TAMs could increase the expression of VEGFA and that inhibition of VEGFA could inhibit the biological function caused by TAMs. Finally, a Western blotting assay was used to evaluate the expression of various factors underlying the mechanism of TAMs. VEGFA, MAPK, P-MAPK, BCL-2, and Snail proteins were found to be overexpressed in co-cultured groups, whereas after VEGFA inhibition, MAPK, P-MAPK, BCL-2, and Snail proteins were found to be significantly downregulated in the radiotherapy group. These study results offer important information regarding the mechanism of radioresistance in esophageal cancer.

Indexed as

ApoptosisEsophageal NeoplasmsNeovascularization, PathologicRadiation ToleranceTumor-Associated MacrophagesVascular Endothelial Growth Factor AAnimalsCell Line, TumorCell MovementCell ProliferationCoculture TechniquesHumansMiceSignal TransductionTHP-1 CellsVascular Endothelial Growth Factor AVEGFA protein, humanAngiogenic pathwayEsophageal cancerRadioresistanceTumor-associated macrophagesVEGF

Identifiers

PMID38772984

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.