Evidence map›Paper›PMID 38771494›Full record

ArticleMolecular biology reports2024

A case control study investigating the methylation levels of GHRL and GHSR genes in alcohol use disorder.

Selin Özkan-Kotiloğlu, Dilek Kaya-Akyüzlü, Emine Güven, Özlem Doğan, Ece Ağtaş-Ertan, İnci Özgür-İlhan

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Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

6 authors.

Selin Özkan-KotiloğluDepartment of Molecular Biology and Genetics, Faculty of Science and Art, Kırşehir Ahi Evran University, Kırşehir, Türkiye. selin.ozkan@ahievran.edu.tr.
Dilek Kaya-AkyüzlüInstitute of Forensic Sciences, Ankara University, Ankara, Türkiye.
Emine GüvenDepartment of Biomedical Engineering, Faculty of Engineering, Düzce University, Düzce, Türkiye.
Özlem DoğanDepartment of Medical Biochemistry, Faculty of Medicine, Ankara University, Ankara, Türkiye.
Ece Ağtaş-ErtanDepartment of Mental Health and Diseases, Faculty of Medicine, Ankara University, Ankara, Türkiye.
İnci Özgür-İlhanDepartment of Mental Health and Diseases, Faculty of Medicine, Ankara University, Ankara, Türkiye.

Funding

Ankara Universitesi 19B0217001
6 · The paper itself

Abstract

backgroundAlcohol use disorder (AUD) is a relapsing disease described as excessive use of alcohol. Evidence of the role of DNA methylation in addiction is accumulating. Ghrelin is an important peptide known as appetite hormone and its role in addictive behavior has been identified. Here we aimed to determine the methylation levels of two crucial genes (GHRL and GHSR) in ghrelin signaling and further investigate the association between methylation ratios and plasma ghrelin levels.

methodsIndividuals diagnosed with (n = 71) and without (n = 82) AUD were recruited in this study. DNA methylation levels were measured through methylation-sensitive high-resolution melting (MS-HRM). Acylated ghrelin levels were detected by ELISA. The GHRL rs696217 polymorphism was analyzed by the standard PCR-RFLP method.

resultsGHRL was significantly hypermethylated (P < 0.0022) in AUD between 25 and 50% methylation than in control subjects but no significant changes of GHSR methylation were observed. Moreover, GHRL showed significant positive correlation of methylation ratio between 25 and 50% with age. A significant positive correlation between GHSR methylation and ghrelin levels in the AUD group was determined (P = 0.037). The level of GHRL methylation and the ghrelin levels showed a significant association in the control subjects (P = 0.042).

conclusionGHSR and GHRL methylation levels did not change significantly between control and AUD groups. However, GHRL and GHSR methylations seemed to have associations with plasma ghrelin levels in two groups. This is the first study investigating the DNA methylation of GHRL and GHSR genes in AUD.

Indexed as

AlcoholismDNA MethylationGhrelinReceptors, GhrelinAdultCase-Control StudiesFemaleHumansMaleMiddle AgedPolymorphism, Single NucleotideGhrelinGHRL protein, humanGhsr1a protein, humanReceptors, GhrelinAddictionAlcohol use disorderDNA methylationGhrelinGrowth hormone secretagogue receptor type A1

Identifiers

PMID38771494

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