Evidence map›Paper›PMID 38771491›Full record

ArticlePlant cell reports2024

Bridging fungal resistance and plant growth through constitutive overexpression of Thchit42 gene in Pelargonium graveolens.

Kahkashan Khatoon, Zafar Iqbal Warsi, Akanksha Singh, Kajal Singh, Feroz Khan, Palak Singh, Rakesh Kumar Shukla, Ram Swaroop Verma, Munmun K Singh, Sanjeet K Verma and 5 more

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Article in Plant cell reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

15 authors.

Kahkashan KhatoonPlant Tissue Culture Lab, Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, 226015, India.
Zafar Iqbal WarsiPlant Tissue Culture Lab, Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, 226015, India.
Akanksha SinghDivision of Crop Production and Protection, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India.
Kajal SinghDivision of Crop Production and Protection, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India.
Feroz KhanTechnology Dissemination and Computational Biology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India.
Palak SinghTechnology Dissemination and Computational Biology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India.
Rakesh Kumar ShuklaPlant Tissue Culture Lab, Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, 226015, India.
Ram Swaroop VermaPhytochemistry Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India.
Munmun K SinghPhytochemistry Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India.
Sanjeet K VermaPlant Tissue Culture Lab, Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, 226015, India.
Zakir HusainPlant Tissue Culture Lab, Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, 226015, India.
Gazala ParweenPlant Tissue Culture Lab, Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, 226015, India.
Pooja SinghPlant Tissue Culture Lab, Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, 226015, India.
Shama AfrozPlant Tissue Culture Lab, Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, 226015, India.
Laiq Ur RahmanPlant Tissue Culture Lab, Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, 226015, India. l.rahman@cimap.res.in.ORCID http://orcid.org/0000-0003-3289-2755

Funding

Central Institute of Medicinal and Aromatic Plants AROMA MISSIONCentral Institute of Medicinal and Aromatic Plants HCP-0007
6 · The paper itself

Abstract

key messageThchit42 constitutive expression for fungal resistance showed synchronisation with leaf augmentation and transcriptome analysis revealed the Longifolia and Zinc finger RICESLEEPER gene is responsible for plant growth and development. Pelargonium graveolens essential oil possesses significant attributes, known for perfumery and aromatherapy. However, optimal yield and propagation are predominantly hindered by biotic stress. All biotechnological approaches have yet to prove effective in addressing fungal resistance. The current study developed transgenic geranium bridging molecular mechanism of fungal resistance and plant growth by introducing cassette 35S::Thchit42. Furthermore, 120 independently putative transformed explants were regenerated on kanamycin fortified medium. Primarily transgenic lines were demonstrated peak pathogenicity and antifungal activity against formidable Colletotrichum gloeosporioides and Fusarium oxysporum. Additionally, phenotypic analysis revealed ~ 2fold increase in leaf size and ~ 2.1fold enhanced oil content. To elucidate the molecular mechanisms for genotypic cause, de novo transcriptional profiles were analyzed to indicate that the auxin-regulated longifolia gene is accountable for augmentation in leaf size, and zinc finger (ZF) RICESLEEPER attributes growth upregulation. Collectively, data provides valuable insights into unravelling the mechanism of Thchit42-mediated crosstalk between morphological and chemical alteration in transgenic plants. This knowledge might create novel opportunities to cultivate fungal-resistant geranium throughout all seasons to fulfil demand.

Indexed as

Disease ResistanceFusariumGene Expression Regulation, PlantPelargoniumPlant LeavesPlants, Genetically ModifiedColletotrichumGeraniumOils, VolatilePlant DiseasesPlant ProteinsOils, VolatilePlant ProteinsBiotic stressFungal resistanceGenetic transformationGenome walkingPathologyPelargonium graveolensTranscriptome

Identifiers

PMID38771491

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.