Evidence map›Paper›PMID 38771358›Full record

Trial reportEuropean journal of applied physiology2024

The biphasic activity of autophagy and heat shock protein response in peripheral blood mononuclear cells following acute resistance exercise in resistance-trained males.

Kurt A Escobar, Trisha A VanDusseldorp, Kelly E Johnson, Matthew Stratton, James J McCormick, Terence Moriarity, Karol Dokladny, Roger A Vaughan, Chad M Kerksick, Len Kravitz and 1 more

Abstract readRandomized Controlled Trial
PubMed Publisher
In one paragraph

Trial report in European journal of applied physiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Kurt A EscobarPhysiology of Sport and Exercise Lab, Department of Kinesiology, California State University, Long Beach, Long Beach, CA, USA. kurt.escobar@csulb.edu.ORCID http://orcid.org/0000-0002-5094-6895
Trisha A VanDusseldorpBonafide Health, LLC p/b JDS Therapeutics, Harrison, NY, USA.
Kelly E JohnsonDepartment of Kinesiology, Coastal Carolina University, Conway, SC, USA.
Matthew StrattonDepartment of Health, Kinesiology, and Sport, University of South Alabama, Mobile, AL, USA.
James J McCormickHuman and Environmental Physiology Research Unit, School of Human Kinetics, University of Ottawa, Ottawa, Canada.
Terence MoriarityDepartment of Kinesiology, University of Northern Iowa, Cedar Falls, USA.
Karol DokladnyDepartment of Internal Medicine, Health Sciences Center, University of New Mexico, Albuquerque, NM, USA.
Roger A VaughanDepartment of Exercise Science, Congdon School of Health Sciences, High Point University, High Point, NC, USA.
Chad M KerksickExercise and Performance Nutrition Laboratory, College of Science, Technology, and Health, Lindenwood University, St. Charles, MO, USA.
Len KravitzDepartment of Health, Exercise, and Sport Science, University of New Mexico, Albuquerque, NM, USA.
Christine M MermierDepartment of Health, Exercise, and Sport Science, University of New Mexico, Albuquerque, NM, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeAutophagy and heat shock protein (HSP) response are proteostatic systems involved in the acute and adaptive responses to exercise. These systems may upregulate sequentially following cellular stress including acute exercise, however, currently few data exist in humans. This study investigated the autophagic and HSP responses to acute intense lower body resistance exercise in peripheral blood mononuclear cells (PBMCs) with and without branched-chain amino acids (BCAA) supplementation.

methodsTwenty resistance-trained males (22.3 ± 1.5 yr; 175.4 ± .7 cm; 86.4 ± 15.6 kg) performed a bout of intense lower body resistance exercise and markers of autophagy and HSP70 were measured immediately post- (IPE) and 2, 4, 24, 48, and 72 h post-exercise. Prior to resistance exercise, 10 subjects were randomly assigned to BCAA supplementation of 0.22 g/kg/d for 5 days pre-exercise and up to 72 h following exercise while the other 10 subjects consumed a placebo (PLCB).

resultsThere were no difference in autophagy markers or HSP70 expression between BCAA and PLCB groups. LC3II protein expression was significantly lower 2 and 4 h post-exercise compared to pre-exercise. LC3II: I ratio was not different at any time point compared to pre-exercise. Protein expression of p62 was lower IPE, 2, and 4 h post-exercise and elevated 24 h post-exercise. HSP70 expression was elevated 48 and 72 h post-exercise.

conclusionsAutophagy and HSP70 are upregulated in PBMCs following intense resistance exercise with autophagy increasing initially post-exercise and HSP response in the latter period. Moreover, BCAA supplementation did not affect this response.

Indexed as

AutophagyLeukocytes, MononuclearResistance TrainingAdultAmino Acids, Branched-ChainDietary SupplementsHSP70 Heat-Shock ProteinsHumansMaleYoung AdultAmino Acids, Branched-ChainHSP70 Heat-Shock ProteinsAutophagyBranched-chain amino acidsHeat shock proteinsProteostasisResistance exerciseTraining

Identifiers

PMID38771358

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.