Evidence map›Paper›PMID 38770143›Full record

ArticleiScience2024

hESC- and hiPSC-derived Schwann cells are molecularly comparable and functionally equivalent.

Kathryn R Moss, Ruifa Mi, Riki Kawaguchi, Jeffrey T Ehmsen, Qiang Shi, Paula I Vargas, Bipasha Mukherjee-Clavin, Gabsang Lee, Ahmet Höke

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Combinatorial expression of glial transcription factors induces Schwann cell-specific gene expression in mouse embryonic fibroblasts.Developmental dynamics : an official publication of the American Association of Anatomists · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Kathryn R MossDepartment of Neurology, Neuromuscular Division, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Ruifa MiDepartment of Neurology, Neuromuscular Division, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Riki KawaguchiSemel Institute for Neuroscience and Human Behavior, University of California, Los Angeles, Los Angeles David Geffen School of Medicine, Los Angeles, CA 90095, USA.
Jeffrey T EhmsenDepartment of Neurology, Neuromuscular Division, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Qiang ShiDepartment of Neurology, Neuromuscular Division, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Paula I VargasDepartment of Neurology, Neuromuscular Division, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Bipasha Mukherjee-ClavinDepartment of Neurology, Neuromuscular Division, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Gabsang LeeDepartment of Neurology, Neuromuscular Division, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Ahmet HökeDepartment of Neurology, Neuromuscular Division, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Funding

Postdoctoral Medical Genetics Training GrantT32GM007471 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI TAO WANG · 1985 to 2026
$8.0M
Function and Regulation of PMP22 in CMT1A and HNPPK22NS125057 · NINDS · UNIVERSITY OF MISSOURI-COLUMBIA · PI MOSS, KATHRYN RENAE · 2021 to 2025
$952k
NIGMS NIH HHS T32 GM007471NINDS NIH HHS K22 NS125057
6 · The paper itself

Abstract

Establishing robust models of human myelinating Schwann cells is critical for studying peripheral nerve injury and disease. Stem cell differentiation has emerged as a key human cell model and disease motivating development of Schwann cell differentiation protocols. Human embryonic stem cells (hESCs) are considered the ideal pluripotent cell but ethical concerns regarding their use have propelled the popularity of human induced pluripotent stem cells (hiPSCs). Given that the equivalence of hESCs and hiPSCs remains controversial, we sought to compare the molecular and functional equivalence of hESC- and hiPSC-derived Schwann cells generated with our previously reported protocol. We identified only modest transcriptome differences by RNA sequencing and insignificant proteome differences by antibody array. Additionally, both cell types comparably improved nerve regeneration and function in a chronic denervation and regeneration animal model. Our findings demonstrate that Schwann cells derived from hESCs and hiPSCs with our protocol are molecularly comparable and functionally equivalent.

Indexed as

Cell biologyNeuroscienceStem cells researchTranscriptomics

Identifiers

PMID38770143
PMCPMC11103364

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.