Evidence map›Paper›PMID 38767355›Full record

ArticleApplied and environmental microbiology2024

High-throughput nanopore DNA sequencing of large insert fosmid clones directly from bacterial colonies.

Léa Chuzel, Amit Sinha, Caileigh V Cunningham, Christopher H Taron

Abstract read
In one paragraph

Article in Applied and environmental microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Léa Chuzel *New England Biolabs, Ipswich, Massachusetts, USA.ORCID 0000-0001-7185-5872
Amit Sinha *New England Biolabs, Ipswich, Massachusetts, USA.
Caileigh V CunninghamNew England Biolabs, Ipswich, Massachusetts, USA.
Christopher H TaronNew England Biolabs, Ipswich, Massachusetts, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fosmids and cosmids are vectors frequently used in functional metagenomic studies. With a large insert capacity (around 30 kb) they can encode dozens of cloned genes or in some cases, entire biochemical pathways. Fosmids with cloned inserts can be transferred to heterologous hosts and propagated to enable screening for new enzymes and metabolites. After screening, fosmids from clones with an activity of interest must be

Indexed as

Escherichia coliHigh-Throughput Nucleotide SequencingNanopore SequencingCloning, MolecularCosmidsDNA, BacterialMetagenomicsNanoporesSequence Analysis, DNADNA, BacterialDNA sequencingfosmidmetagenomicsnanopore

Identifiers

PMID38767355
PMCPMC11218629

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.