Evidence map›Paper›PMID 38766149›Full record

ArticlebioRxiv : the preprint server for biology2024

A series of spontaneously blinking dyes for super-resolution microscopy.

Katie L Holland, Sarah E Plutkis, Timothy A Daugird, Abhishek Sau, Jonathan B Grimm, Brian P English, Qinsi Zheng, Sandeep Dave, Fariha Rahman, Liangqi Xie and 9 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

19 authors.

Katie L HollandJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID 0009-0007-1183-195X
Sarah E PlutkisJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID 0009-0003-1822-0466
Timothy A DaugirdDepartment of Pharmacology, University of North Carolina-Chapel Hill Medical School, Chapel Hill, NC, USA.ORCID 0000-0002-6313-0246
Abhishek SauDepartment of Cell Biology and Genetics, Texas A&M University, School of Medicine, College Station, TX, USA.ORCID 0000-0002-5560-5360
Jonathan B GrimmJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID 0000-0003-0331-4200
Brian P EnglishJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID 0000-0002-4037-6294
Qinsi ZhengJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID 0000-0003-0348-9721
Sandeep DaveDepartment of Cell Biology and Genetics, Texas A&M University, School of Medicine, College Station, TX, USA.ORCID 0000-0003-2832-2098
Fariha RahmanJoint Department of Biomedical Engineering, University of North Carolina-Chapel Hill and North Carolina State University, Chapel Hill, NC, USA.
Liangqi XieJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID 0000-0002-6546-9622
Peng DongJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Ariana N TkachukJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID 0000-0001-8991-9643
Timothy A BrownJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID 0000-0001-8941-7678
Robert H SingerJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID 0000-0002-6725-0093
Zhe LiuJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID 0000-0002-3592-3150
Catherine G GalbraithDepartment of Biomedical Engineering and Knight Cancer Institute, Oregon Health and Science University, Portland, OR, USA.ORCID 0000-0003-0203-1487
Siegfried M MusserDepartment of Cell Biology and Genetics, Texas A&M University, School of Medicine, College Station, TX, USA.ORCID 0000-0002-7793-2557
Wesley R LegantDepartment of Pharmacology, University of North Carolina-Chapel Hill Medical School, Chapel Hill, NC, USA.ORCID 0000-0003-1827-5371
Luke D LavisJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID 0000-0002-0789-6343

Funding

Mechanism of MRNA Localization and Localized Translation in NeuronsR01NS083085 · NINDS · ALBERT EINSTEIN COLLEGE OF MEDICINE, INC · PI Sulagna Das, Robert H Singer · 2013 to 2026
$9.0M
Mapping Transport Pathways through Nuclear Pores using 3D Super-Resolution MicroscopyR01GM126190 · NIGMS · TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR · PI MUSSER, SIEGFRIED M · 2018 to 2025
$2.6M
Connecting the dots between single molecule dynamics and cell differentiationDP2GM136653 · NIGMS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI LEGANT, WESLEY R. · 2019 to 2019
$2.3M
Molecular Mechanisms of Cell MigrationR01GM117188 · NIGMS · OREGON HEALTH & SCIENCE UNIVERSITY · PI GALBRAITH, CATHERINE GREEN · 2018 to 2022
$1.8M
NIGMS NIH HHS DP2 GM136653NIGMS NIH HHS R01 GM117188NIGMS NIH HHS R01 GM126190NINDS NIH HHS R01 NS083085
6 · The paper itself

Abstract

Spontaneously blinking fluorophores permit the detection and localization of individual molecules without reducing buffers or caging groups, thus simplifying single-molecule localization microscopy (SMLM). The intrinsic blinking properties of such dyes are dictated by molecular structure and modulated by environment, which can limit utility. We report a series of tuned spontaneously blinking dyes with duty cycles that span two orders of magnitude, allowing facile SMLM in cells and dense biomolecular structures.

Identifiers

PMID38766149
PMCPMC11101128

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.