Evidence map›Paper›PMID 38766009›Full record

ArticleResearch square2024

Cystatin M/E ameliorates bone resorption through increasing osteoclastic cell estrogen influx.

Dongzheng Gai, Perry C Caviness, Oxana P Lazarenko, Jennifer F Chen, Christopher E Randolph, Zijun Zhang, Yan Cheng, Fumou Sun, Hongwei Xu, Michael L Blackburn and 4 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Dongzheng GaiMyeloma Center, Winthrop P. Rockefeller Cancer Institute, Department of Internal Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.
Perry C CavinessArkansas Children's Nutrition Center, Little Rock, AR 72205, USA.
Oxana P LazarenkoArkansas Children's Nutrition Center, Little Rock, AR 72205, USA.
Jennifer F ChenUndergraduate Pre-Medical Program, University of Arkansas at Fayetteville, Fayetteville, AR, 72701, USA.
Christopher E RandolphCenter for Translational Pediatric Research, Arkansas Children's Research Institute, Little Rock, AR 72202, USA.
Zijun ZhangMyeloma Center, Winthrop P. Rockefeller Cancer Institute, Department of Internal Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.
Yan ChengMyeloma Center, Winthrop P. Rockefeller Cancer Institute, Department of Internal Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.
Fumou SunMyeloma Center, Winthrop P. Rockefeller Cancer Institute, Department of Internal Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.
Hongwei XuMyeloma Center, Winthrop P. Rockefeller Cancer Institute, Department of Internal Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.
Michael L BlackburnArkansas Children's Nutrition Center, Little Rock, AR 72205, USA.
Guido TricotMyeloma Center, Winthrop P. Rockefeller Cancer Institute, Department of Internal Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.
John D ShaughnessyMyeloma Center, Winthrop P. Rockefeller Cancer Institute, Department of Internal Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.
Jin-Ran ChenArkansas Children's Nutrition Center, Little Rock, AR 72205, USA.ORCID 0000-0001-6984-5878
Fenghuang ZhanMyeloma Center, Winthrop P. Rockefeller Cancer Institute, Department of Internal Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.

Funding

Prevention of MGUS Progression to MM by Modulating the Bone Marrow MicroenvironmentU54CA272691 · NCI · CHILDREN'S MERCY HOSP (KANSAS CITY, MO) · PI FENGHUANG ZHAN · 2023 to 2026
$6.1M
The role of oxidative stress in alcohol-induced osteopeniaR37AA018282 · NIAAA · LSU HEALTH SCIENCES CENTER · PI RONIS, MARTIN J J · 2016 to 2025
$4.7M
Towards a better understanding of the critical role and therapeutic potential of drug resistant genes in multiple myelomaR01CA236814 · NCI · UNIV OF ARKANSAS FOR MED SCIS · PI FENGHUANG ZHAN · 2020 to 2026
$2.5M
Automated Problem and Allergy Lists Enrichment Based on High Accuracy Information Extraction from the Electronic Health RecordR42CA180190 · NCI · CLINACUITY,INC. · PI MEYSTRE, STEPHANE · 2016 to 2017
$1.5M
Automated Dynamic Lists for Efficient Electronic Health Record ManagementR41CA180190 · NCI · CLINACUITY,INC. · PI MEYSTRE, STEPHANE · 2013 to 2014
$422k
NCI NIH HHS R01 CA236814NCI NIH HHS R41 CA180190NCI NIH HHS R42 CA180190NCI NIH HHS U54 CA272691NIAAA NIH HHS R37 AA018282
6 · The paper itself

Abstract

In multiple myeloma (MM), increased osteoclast differentiation leads to the formation of osteolytic lesions in most MM patients. Bisphosphonates, such as zoledronic acid (ZA), are used to ameliorate bone resorption, but due to risk of serious side effects as well as the lack of repair of existing lesions, novel anti-bone resorption agents are required. Previously, the absence of osteolytic lesions in MM was strongly associated with elevated levels of cystatin M/E (CST6), a cysteine protease inhibitor, secreted by MM cells. In this study, both MM- and ovariectomy (OVX)-induced osteoporotic mouse models were used to compare the effects of recombinant mouse CST6 (rmCst6) and ZA on preventing bone loss. μCT showed that rmCst6 and ZA had similar effects on improving percent bone volume, and inhibited differentiation of non-adherent bone marrow cells into mature osteoclasts. Single-cell RNA sequencing showed that rmCst6 and not ZA treatment reduced bone marrow macrophage percentage in the MM mouse model compared to controls. Protein and mRNA arrays showed that both rmCst6 and ZA significantly inhibit OVX-induced expression of inflammatory cytokines. For OVX mice, ERα protein expression in bone was brought to sham surgery level by only rmCst6 treatments. rmCst6 significantly increased mRNA and protein levels of ERα and significantly increased total intracellular estrogen concentrations for

Identifiers

PMID38766009
PMCPMC11100902

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.