Evidence map›Paper›PMID 38765969›Full record

ArticlebioRxiv : the preprint server for biology2024

Domain consolidation in Bacterial 50S assembly revealed by Anti-Sense Oligonucleotide Probing.

Kai Sheng, Xiyu Dong, Sriram Aiyer, Joan Lee, Selena Djordjevic Marquardt, Dmitry Lyumkis, James R Williamson

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Kai ShengDepartment of Integrative Structural and Computational Biology, Department of Chemistry, and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.ORCID 0000-0001-9394-2256
Xiyu DongDepartment of Integrative Structural and Computational Biology, Department of Chemistry, and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.ORCID 0000-0002-1766-3324
Sriram AiyerLaboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.ORCID 0000-0001-6436-0521
Joan LeeDepartment of Integrative Structural and Computational Biology, Department of Chemistry, and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.ORCID 0009-0002-3573-2167
Selena Djordjevic MarquardtLaboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.ORCID 0000-0003-0170-5304
Dmitry LyumkisDepartment of Integrative Structural and Computational Biology, Department of Chemistry, and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.ORCID 0000-0002-8124-7472
James R WilliamsonDepartment of Integrative Structural and Computational Biology, Department of Chemistry, and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.ORCID 0000-0002-8772-468X

Funding

Structural Biology CoreU54AI170855 · NIAID · SEATTLE CHILDREN'S HOSPITAL · PI Karen A Kirby · 2022 to 2026
$36.7M
Dynamics of the Bacterial Ribosome and Proteome (Equipment Supplement)R35GM136412 · NIGMS · SCRIPPS RESEARCH INSTITUTE, THE · PI James R Williamson · 2020 to 2026
$4.9M
NIAID NIH HHS U54 AI170855NIGMS NIH HHS R35 GM136412
6 · The paper itself

Abstract

Investigating the intricate and rapid folding kinetics of large RNA-protein complexes (RNPs), like the bacterial ribosome, remains a formidable challenge in structural biology. Previous genetic approaches to probe assembly have focused on modulating the expression of either r-proteins or assembly factors. Here, anti-sense oligonucleotides (ASOs) were used to disrupt native RNA/RNA and RNA/protein interactions, in order to generate novel folding intermediates. In an

Identifiers

PMID38765969
PMCPMC11100757

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.