Evidence map›Paper›PMID 38765530›Full record

ArticleRevista brasileira de ginecologia e obstetricia : revista da Federacao Brasileira das Sociedades de Ginecologia e Obstetricia2024

Timing of semen cryopreservation: before or after processing?

Ana Paula de Souza Kussler, Ivan Cunha Bustamante, Elisa Negri, Edison Capp, Helena von Eye Corleta

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Revista brasileira de ginecologia e obstetricia : revista da Federacao Brasileira das Sociedades de Ginecologia e Obstetricia, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ana Paula de Souza KusslerUniversidade Federal do Rio Grande do Sul Faculdade de Medicina Porto AlegreRS Brazil Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.ORCID https://orcid.org/0000-0002-2492-1694
Ivan Cunha BustamanteUniversidade do Vale do Taquari LajeadoRS Brazil Universidade do Vale do Taquari, Lajeado, RS, Brazil.ORCID https://orcid.org/0000-0002-0793-8443
Elisa NegriUniversidade Federal do Rio Grande do Sul Faculdade de Medicina Porto AlegreRS Brazil Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.ORCID https://orcid.org/0000-0002-5266-8606
Edison CappUniversidade Federal do Rio Grande do Sul Faculdade de Medicina Porto AlegreRS Brazil Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.ORCID https://orcid.org/0000-0002-1039-7940
Helena von Eye CorletaUniversidade Federal do Rio Grande do Sul Faculdade de Medicina Porto AlegreRS Brazil Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.ORCID https://orcid.org/0000-0001-7406-737X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: Seminal cryopreservation causes significant damage to the sperm; therefore, different methods of cryopreservation have been studied. The aim of the study was to compare the effects of density gradient processing and washing/centrifugation with seminal plasma removal for cryopreservation in semen parameters. Methods: Seminal samples of 26 normozoospermic patients were divided into 3 parts: with seminal plasma; after washing/centrifugation; and after selection through density gradient. The samples were cryopreserved for at least two weeks. Motility, sperm count, morphology and viability were evaluated before cryopreservation and after thawing. Results: Density gradient processing selected motile and viable sperm with normal morphology in fresh samples (p<0.05). Cryopreservation negatively affected all sperm parameters regardless of the processing performed, and even if the sperm recovery was lower in the density gradient after the thawing, progressive motility, total motility, viability and morphology remained higher (p<0.05). Conclusion: Cryopreservation significantly compromises sperm parameters (motility, morphology, viability). In normozoospermic patients, the density gradients select better quality spermatozoa compared to other processing methods; this benefit was kept after thawing.

Indexed as

CryopreservationSemen PreservationAdultHumansMaleSemenSemen AnalysisSperm MotilityTime FactorsCentrifugationCryopreservationDensity gradientSemenSeminal plasmaSpermatozoaSperm countSperm wash

Identifiers

PMID38765530
PMCPMC11075422

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.