Evidence map›Paper›PMID 38763479›Full record

ArticleDevelopmental and comparative immunology2024

TLR7 in channel catfish (Ictalurus punctatus) is expressed in the endolysosome and is stimulated by synthetic ssRNA analogs, imiquimod, and resiquimod.

Kristianna L Felch, Jonathan D Crider, Debduti Bhattacharjee, Cameron Huhn, Melanie Wilson, Eva Bengtén

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental and comparative immunology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Kristianna L FelchDepartment of Cell and Molecular Biology, University of Mississippi Medical Center, 2500 North State Street, 39216, Jackson, MS, USA. Electronic address: ksaelens@umc.edu.
Jonathan D CriderDepartment of Cell and Molecular Biology, University of Mississippi Medical Center, 2500 North State Street, 39216, Jackson, MS, USA; Department of Biology, Belmont University, 1900 Belmont Blvd, 37212, Nashville, TN, USA. Electronic address: jonathan.crider@belmont.edu.
Debduti BhattacharjeeDepartment of Cell and Molecular Biology, University of Mississippi Medical Center, 2500 North State Street, 39216, Jackson, MS, USA. Electronic address: debduti.bhattacharjee@mnsu.edu.
Cameron HuhnDepartment of Cell and Molecular Biology, University of Mississippi Medical Center, 2500 North State Street, 39216, Jackson, MS, USA. Electronic address: chuhn1@umc.edu.
Melanie WilsonDepartment of Cell and Molecular Biology, University of Mississippi Medical Center, 2500 North State Street, 39216, Jackson, MS, USA; Center for Immunology and Microbial Research, University of Mississippi Medical Center, 2500 North State Street, 39216, Jackson, MS, USA. Electronic address: mwilson@umc.edu.
Eva BengténDepartment of Cell and Molecular Biology, University of Mississippi Medical Center, 2500 North State Street, 39216, Jackson, MS, USA; Center for Immunology and Microbial Research, University of Mississippi Medical Center, 2500 North State Street, 39216, Jackson, MS, USA. Electronic address: ebengten@umc.edu.

Funding

Role of obesity in preeclamptic pregnancy.P20GM121334 · NIGMS · UNIVERSITY OF MISSISSIPPI MED CTR · PI Babbette LaMarca, Jane F Reckelhoff · 2017 to 2026
$26.4M
NIGMS NIH HHS P20 GM121334
6 · The paper itself

Abstract

Toll-like receptors (TLRs) are pivotal pattern recognition receptors (PRRs) and key mediators of innate immunity. Despite the significance of channel catfish (Ictalurus punctatus) in comparative immunology and aquaculture, its 20 TLR genes remain largely functionally uncharacterized. In this study, our aim was to determine the catfish TLR7 agonists, signaling potential, and cellular localization. Using a mammalian reporter system, we identified imiquimod and resiquimod, typical ssRNA analogs, as potent catfish TLR7 agonists. Notably, unlike grass carp TLR7, catfish TLR7 lacks the ability to respond to poly (I:C). Confocal microscopy revealed predominant catfish TLR7 expression in lysosomes, co-localizing with the endosomal chaperone protein, UNC93B1. Furthermore, imiquimod stimulation elicited robust IFNb transcription in peripheral blood leukocytes isolated from adult catfish. These findings underscore the conservation of TLR7 signaling in catfish, reminiscent of mammalian TLR7 responses. Our study sheds light on the functional aspects of catfish TLR7 and contributes to a better understanding of its role in immune defense mechanisms.

Indexed as

Fish ProteinsIctaluridaeImidazolesImiquimodImmunity, InnateLysosomesToll-Like Receptor 7AminoquinolinesAnimalsHumansPoly I-CSignal TransductionAminoquinolinesFish ProteinsImidazolesImiquimodPoly I-CresiquimodToll-Like Receptor 7

Identifiers

PMID38763479
PMCPMC11234115

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.