Evidence map›Paper›PMID 38763350›Full record

ArticleCryobiology2024

Cholesterol-loaded cyclodextrin improves motility and survival of cryopreserved zebrafish (Danio rerio) sperm.

Jennifer L Matthews, Joy M Murphy, Zoltan M Varga

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cryobiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jennifer L MatthewsZebrafish International Resource Center, 5274 University of Oregon, Eugene, OR, 97403, USA.
Joy M MurphyZebrafish International Resource Center, 5274 University of Oregon, Eugene, OR, 97403, USA.
Zoltan M VargaZebrafish International Resource Center, 5274 University of Oregon, Eugene, OR, 97403, USA. Electronic address: zoltan@zebrafish.org.

Funding

Zebrafish International Resource Center (ZIRC)P40OD011021 · OD · UNIVERSITY OF OREGON · PI Zoltan Marton Varga · 2012 to 2026
$36.0M
NIH HHS P40 OD011021
6 · The paper itself

Abstract

We studied the impact of modulating cholesterol levels in zebrafish sperm plasma membranes using cholesterol-loaded methyl-β-cyclodextrin (CLC) and unloaded methyl-β-cyclodextrin (MβC). Zebrafish sperm were treated with these substances before cryopreservation, and post-thaw sperm motility and in vitro fertilization (IVF) rates were compared between treated and untreated samples. Our findings indicate that adding cholesterol to sperm membranes increases post-thaw motility, motile cell count, and motile cell survival within a 0.5-4.0 mg per 1.2 × 10

Indexed as

beta-CyclodextrinsCell SurvivalCholesterolCryopreservationCryoprotective AgentsSemen PreservationSpermatozoaSperm MotilityZebrafishAnimalsCell MembraneFertilization in VitroMalebeta-CyclodextrinsCholesterolCryoprotective Agentsmethyl-beta-cyclodextrinCholesterolCryobankingCryopreservationMethodMethyl-β-cyclodextrinProtocolQuality assessmentQuality control

Identifiers

PMID38763350
PMCPMC11374480

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.