ArticleThe Plant journal : for cell and molecular biology2024
In planta imaging of pyridine nucleotides using second-generation fluorescent protein biosensors.
Article in The Plant journal : for cell and molecular biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Spatiotemporal Dynamics of Cytosolic NADPH in Living Arabidopsis thaliana.Plant, cell & environment · 2026Article
- PERSPECTIVE: organelle positioning as a principle of metabolic regulation and stress tolerance.The Plant journal : for cell and molecular biology · 2026Review
- A family of NADPH/NADPNature communications · 2024Article
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Authors and funding
9 authors.
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Abstract
Redox changes of pyridine nucleotides in cellular compartments are highly dynamic and their equilibria are under the influence of various reducing and oxidizing reactions. To obtain spatiotemporal data on pyridine nucleotides in living plant cells, typical biochemical approaches require cell destruction. To date, genetically encoded fluorescent biosensors are considered to be the best option to bridge the existing technology gap, as they provide a fast, accurate, and real-time readout. However, the existing pyridine nucleotides genetically encoded fluorescent biosensors are either sensitive to pH change or slow in dissociation rate. Herein, we employed the biosensors which generate readouts that are pH stable for in planta measurement of NADH/NAD
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