ArticleScientific reports2024
Evaluation of DNA yield from various tissue and sampling sources for use in single nucleotide polymorphism panels.
Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- Umbilical Cord: A Non-Invasive DNA Source for Sea Turtle Genomics.Molecular ecology resources · 2026Article
- A Practical Framework for GT-Seq Panel Optimization.Molecular ecology resources · 2026Article
- The yields of nucleic acids and proteins extracted from various murine tissue types.Acta biochimica Polonica · 2026Article
- Exploring the presence of the GPR126 single nucleotide polymorphism (rs536714306) in periodontal patients of European ancestry.BMC research notes · 2025Article
- Reducing DNA extraction costs through factorial design for the DNAdvance Kit.BMC research notes · 2024Article
- Evaluation of DNA yield from various tissue and sampling sources for use in single nucleotide polymorphism panels.Scientific reports · 2024Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Genetics studies are used by wildlife managers and researchers to gain inference into a population of a species of interest. To gain these insights, microsatellites have been the primary method; however, there currently is a shift from microsatellites to single nucleotide polymorphisms (SNPs). With the different DNA requirements between microsatellites and SNPs, an investigation into which samples can provide adequate DNA yield is warranted. Using samples that were collected from previous genetic projects from regions in the USA from 2014 to 2021, we investigated the DNA yield of eight sample categories to gain insights into which provided adequate DNA to be used in ddRADseq or already developed high- or medium-density SNP panels. We found seven sample categories that met the DNA requirements for use in all three panels, and one sample category that did not meet any of the three panels requirements; however, DNA integrity was highly variable and not all sample categories that met panel DNA requirements could be considered high quality DNA. Additionally, we used linear random-effects models to determine which covariates would have the greatest influence on DNA yield. We determined that all covariates (tissue type, storage method, preservative, DNA quality, time until DNA extraction and time after DNA extraction) could influence DNA yield.
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Registered trials
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