In Vitro Titan Cell Generation in Cryptococcus neoformans and Automated Cell Size Measurements.

Benjamin Hommel, Aude Sturny-Leclère, Alexandre Alanio

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

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2 · The registry

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

3 authors.

Benjamin HommelTranslational Mycology Research Group, Institut Pasteur, Université Paris Cité, Centre National de Référence Mycoses Invasives et Antifongiques, Département de Mycologie, Paris, France.

Aude Sturny-LeclèreInstitut Pasteur, Université Paris Cité,National Reference Center for Invasive Mycoses and Antifungals, Paris, France.

Alexandre AlanioTranslational Mycology Research Group, Institut Pasteur, Université Paris Cité, Centre National de Référence Mycoses Invasives et Antifongiques, Département de Mycologie, Paris, France. alexandre.alanio@pasteur.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A special feature of the human fungal pathogen Cryptococcus neoformans is its morphological changes triggered by the interaction with the host. During infection, a specific increase in cell size is observed, particularly in lung tissue, from a typical cell size of 5-7 μm cells to cells larger than 10 μm, dubbed titan cells (TCs). However, the study of this specific cell subpopulation was, until now, only possible via recovery of TCs from lungs of mice during experimental infections where stable and reproducible generation of TCs occurs.The protocol described here generates TCs using in vitro conditions and measures cell size using a rapid, automated method. TC generation in vitro is robust and reproducible, generating yeast cells harboring the same characteristics of TCs generated in vivo.

Indexed as

Cryptococcus neoformansAnimalsCell SizeCryptococcosisHumansLungMiceCell size countingCryptococcosisCryptococcus neoformansIn vitroTitan cells

Identifiers

PMID38758332

DOI10.1038/nmeth.2075

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.