Evidence map›Paper›PMID 38758325›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Measuring Stress Phenotypes in Cryptococcus neoformans.

Rajendra Upadhya, Corinna Probst, J Andrew Alspaugh, Jennifer K Lodge

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Acetylation and accessibility ofCell surface (Amsterdam, Netherlands) · 2026
    Article
  6. Article
  7. Article
  8. Cryptococcal nutrient acquisition and pathogenesis: dining on the host.Microbiology and molecular biology reviews : MMBR · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Rajendra UpadhyaDepartment of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC, USA. rajendra.upadhya@duke.edu.
Corinna ProbstDepartment of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC, USA.
J Andrew AlspaughDepartment of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC, USA.
Jennifer K LodgeDepartment of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC, USA.

Funding

Preclinical studies of a Cryptococcus vaccine for AIDS patientsR01AI125045 · NIAID · WASHINGTON UNIVERSITY · PI LEVITZ, STUART MICHAEL, LODGE, JENNIFER K. · 2016 to 2025
$7.1M
Chitosan in CryptococcusR01AI072195 · NIAID · WASHINGTON UNIVERSITY · PI LODGE, JENNIFER K. · 2008 to 2018
$3.5M
NIAID NIH HHS R01 AI072195NIAID NIH HHS R01 AI125045
6 · The paper itself

Abstract

Cryptococcus neoformans is an opportunistic human fungal pathogen capable of surviving in a wide range of environments and hosts. It has been developed as a model organism to study fungal pathogenesis due to its fully sequenced haploid genome and optimized gene deletion and mutagenesis protocols. These methods have greatly aided in determining the relationship between Cryptococcus genotype and phenotype. Furthermore, the presence of congenic mata and matα strains associated with a defined sexual cycle has helped further understand cryptococcal biology. Several in vitro stress conditions have been optimized to closely mimic the stress that yeast encounter in the environment or within the infected host. These conditions have proven to be extremely useful in elucidating the role of several genes in allowing yeast to adapt and survive in hostile external environments. This chapter describes various in vitro stress conditions that could be used to test the sensitivity of different mutant strains, as well as the protocol for preparing them. We have also included a list of mutants that could be used as a positive control strain when testing the sensitivity of the desired strain to a specific stress.

Indexed as

Cryptococcus neoformansPhenotypeStress, PhysiologicalCryptococcosisHumansMutationAlkaline stressCapsuleCell wall stressCryptococcus growthCryptococcus growthMelaninNitrosative stressOxidative stressTemperature stress

Identifiers

PMID38758325
PMCPMC11521573

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.