Evidence map›Paper›PMID 38758320›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Two Methods of Measuring Cryptococcus neoformans Fungal Burden in Macrophages.

Emily Smith-Peavler, Linda M Sircy, David E Nelson, Erin E McClelland

Abstract read
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Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Emily Smith-Peavler *Department of Biology, Middle Tennessee State University, Murfreesboro, TN, USA.
Linda M Sircy *Department of Biology, Middle Tennessee State University, Murfreesboro, TN, USA.
David E NelsonDepartment of Biology, Middle Tennessee State University, Murfreesboro, TN, USA.
Erin E McClellandDepartment of Biomedical Sciences, Marian University College of Osteopathic Medicine, Indianapolis, IN, USA. emcclelland@marian.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The ability of C. neoformans to survive and replicate within host phagocytes enables it to evade the immune system and allows for persistence of the infection. As such, measuring fungal burden of C. neoformans strains-and indeed how drug treatments can influence fungal burden-provides important information about C. neoformans pathogenesis. In this chapter, we describe two methods that may be used to appraise fungal burden: a standard end-point colony-formation assay for calculating the average number of yeast per host cell and a fluorescence microscopy-based method that may be used to measure changes in fungal burden in individual living macrophages in real time.

Indexed as

CryptococcosisCryptococcus neoformansMacrophagesMicroscopy, FluorescenceAnimalsColony Count, MicrobialHumansMiceCryptococcus neoformansFungal burdenIntracellular fungal pathogenLive cell imagingMacrophage cell linesPhagocytosis assays

Identifiers

PMID38758320

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.