Evidence map›Paper›PMID 38758318›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Interaction Between Macrophages and Cryptococcus neoformans: Distinguishing Phagocytosed Versus External Fungi.

Amanda Pereira Rocha, Thaís Silva Alves, Adrielle Veloso Caixeta, Patrícia Albuquerque, André Moraes Nicola

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Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Amanda Pereira RochaFaculty of Medicine, University of Brasília, Brasilia, DF, Brazil.
Thaís Silva AlvesFaculty of Medicine, University of Brasília, Brasilia, DF, Brazil.
Adrielle Veloso CaixetaFaculty of Medicine, University of Brasília, Brasilia, DF, Brazil.
Patrícia AlbuquerqueFaculty of Ceilândia, University of Brasília, Brasilia, DF, Brazil.
André Moraes NicolaFaculty of Medicine, University of Brasília, Brasilia, DF, Brazil. amnicola@unb.br.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The interaction between macrophages and Cryptococcus neoformans is crucial in the pathogenesis of cryptococcosis. These phagocytes are important immune effectors, but also a niche in which facultative intracellular parasites, such as C. neoformans, thrive. Consequently, phagocytosis of cryptococcal cells and its outcomes are very frequently studied. One major issue with several of the tests used for this, however, is that macrophage-C. neoformans interaction does not always result in phagocytosis, as fungi may be attached to the external surface of the phagocyte. The most used methodologies to study phagocytosis of cryptococcal cells have varying degrees of precision in separating fungi that are truly internalized from those that are outside macrophages. Here we describe two assays to measure phagocytosis that can differentiate internal from external C. neoformans cells.

Indexed as

CryptococcosisCryptococcus neoformansMacrophagesPhagocytosisAnimalsHost-Pathogen InteractionsHumansMiceCryptococcosisCryptococcus neoformansFlow cytometryFluorescence microscopyMacrophagePhagocytosis

Identifiers

PMID38758318

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.