Evidence map›Paper›PMID 38756702›Full record

ArticleRegenerative therapy2024

Scanning electron microscopy analysis of synovial and adipose mesenchymal stem cells adhering to cartilage.

Yusuke Fuchioka, Kentaro Endo, Yuriko Sakamaki, Takahiro Tanimoto, Nobutake Ozeki, Yusuke Nakagawa, Hideyuki Koga, Makoto Tomita, Ichiro Sekiya

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Regenerative therapy, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yusuke FuchiokaCenter for Stem Cell and Regenerative Medicine, Tokyo Medical and Dental University, Tokyo, Japan.
Kentaro EndoCenter for Stem Cell and Regenerative Medicine, Tokyo Medical and Dental University, Tokyo, Japan.
Yuriko SakamakiResearch Core, Tokyo Medical and Dental University, Tokyo, Japan.
Takahiro TanimotoCenter for Stem Cell and Regenerative Medicine, Tokyo Medical and Dental University, Tokyo, Japan.
Nobutake OzekiCenter for Stem Cell and Regenerative Medicine, Tokyo Medical and Dental University, Tokyo, Japan.
Yusuke NakagawaDepartment of Joint Surgery and Sports Medicine, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Hideyuki KogaDepartment of Joint Surgery and Sports Medicine, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Makoto TomitaSchool of Data Science, Graduate School of Data Science, Yokohama City University, Kanagawa, Japan.
Ichiro SekiyaCenter for Stem Cell and Regenerative Medicine, Tokyo Medical and Dental University, Tokyo, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Mesenchymal stem cells (MSCs) are increasingly used for intra-articular injections in the treatment of knee osteoarthritis. The aim of this study was to use scanning electron microscopy (SEM) to compare the morphological characteristics of synovial and adipose MSCs. Methods: Synovium and adipose tissues were concurrently harvested from eight patients with knee osteoarthritis. Suspensions of both synovial and adipose MSCs were examined to identify the presence of microspikes. In addition to this study, the MSC suspensions in four patients were applied to abraded porcine cartilage discs and observed 10 s, 10 min, and 1 h later. Results: The median percentage of cells exhibiting microspikes was 14% for synovial MSC suspensions and 13% for adipose MSC suspensions; this difference was not statistically significant (n = 8). No notable differences were detected in the number of adherent cells or in the proportion of cells displaying microspikes or pseudopodia. Strong correlations were found between the proportion of cells with pseudopodia and the number of attached cells for both synovial (r = 0.92, n = 12) and adipose (r = 0.86, n = 12) MSCs, with no significant difference in the correlation coefficients between the two groups. Conclusion: SEM analysis revealed no obvious differences in morphological characteristics during MSC adhesion to cartilage for either synovial or adipose MSCs.

Indexed as

Adipose tissueKneeMesenchymal stem cellOsteoarthritisScanning electron microscopySynovium

Identifiers

PMID38756702
PMCPMC11096720

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.