Evidence map›Paper›PMID 38755577›Full record

Observational studyBMC gastroenterology2024

Immunohistochemistry and real-time Polymerase Chain Reaction: importance in the diagnosis of intestinal tuberculosis in a Peruvian population.

Fernando Arevalo, Soledad Rayme, Rocío Ramírez, Romy Rolando, Jaime Fustamante, Mario Monteghirfo, Rocio Chavez, Eduardo Monge

Abstract readObservational Study
PubMed Full text at PMC Publisher
In one paragraph

Observational study in BMC gastroenterology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Fernando ArevaloPathology Department, Hospital Nacional Daniel A. Carrión, Callao, Lima, Perú. histodiagnostico1303@hotmail.com.
Soledad RaymePathology Department, Hospital Nacional Daniel A. Carrión, Callao, Lima, Perú.
Rocío RamírezPathology Department, Hospital Nacional Daniel A. Carrión, Callao, Lima, Perú.
Romy RolandoInstituto de Medicina Legal y Ciencias Forenses - Perú, Lima, Perú.
Jaime FustamanteGastroenterology Department, Hospital Nacional Daniel A., Carrión, Lima, Perú.
Mario MonteghirfoDepartamento de Ciencias Dinámicas, Facultad de Medicina, Instituto de Investigacion de Bioquímica y Nutrición Alberto Guzmán Barrón, Universidad Nacional Mayor de San Marcos, Lima, Perú.
Rocio ChavezGastroenterology Department, Hospital Nacional Adolfo Guevara Velasco EsSalud, Cuzco, Perú.
Eduardo MongeGastroenterology Department, Hospital Nacional Daniel A., Carrión, Lima, Perú.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionThe diagnosis of intestinal tuberculosis is challenging even nowadays. This study aims to report the positivity rates of new diagnostic methods such as immunohistochemistry and Real-Time Polymerase Chain Reaction in patients with intestinal tuberculosis, as well as describe the pathological and endoscopic features of intestinal tuberculosis in our population.

methodsThis was a retrospective observational study conducted in patients diagnosed with intestinal tuberculosis, between 2010 to 2023 from the Hospital Nacional Daniel Alcides Carrion and a Private Pathology Center, both located in Peru. Clinical data was obtained, histologic features were independently re-evaluated by three pathologists; and immunohistochemistry and real-time Polymerase Chain Reaction evaluation were performed. The 33 patients with intestinal tuberculosis who fulfilled the inclusion criteria were recruited.

resultsImmunohistochemistry was positive in 90.9% of cases, while real-time Polymerase Chain Reaction was positive in 38.7%. The ileocecal region was the most affected area (33.3%), and the most frequent endoscopic appearance was an ulcer (63.6%). Most of the granulomas were composed solely of epithelioid histiocytes (75.8%). Crypt architectural disarray was the second most frequent histologic finding (78.8%) after granulomas, but most of them were mild.

conclusionSince immunohistochemistry does not require an intact cell wall, it demonstrates higher sensitivity compared to Ziehl-Neelsen staining. Therefore, it could be helpful for the diagnosis of paucibacillary tuberculosis.

Indexed as

ImmunohistochemistryReal-Time Polymerase Chain ReactionTuberculosis, GastrointestinalAdolescentAdultAgedFemaleGranulomaHumansMaleMiddle AgedMycobacterium tuberculosisPeruRetrospective StudiesSensitivity and SpecificityYoung AdultColonoscopyDiagnosisEndoscopic findingsHistologic findingsImmunohistochemistryIntestinal tuberculosisMycobacterium tuberculosisReal-time Polymerase Chain Reaction

Identifiers

PMID38755577
PMCPMC11097500

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.