Evidence map›Paper›PMID 38755524›Full record

ArticleBMC microbiology2024

A novel family of defensin-like peptides from Hermetia illucens with antibacterial properties.

Leila Fahmy, Tomas Generalovic, Youssif M Ali, David Seilly, Kesavan Sivanesan, Lajos Kalmar, Miha Pipan, Graham Christie, Andrew J Grant

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Review
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Leila FahmyDepartment of Veterinary Medicine, University of Cambridge, Cambridge, UK.
Tomas GeneralovicBetter Origin, Future Business Centre, Cambridge, UK.
Youssif M AliDepartment of Veterinary Medicine, University of Cambridge, Cambridge, UK.
David SeillyDepartment of Veterinary Medicine, University of Cambridge, Cambridge, UK.
Kesavan SivanesanDepartment of Veterinary Medicine, University of Cambridge, Cambridge, UK.
Lajos KalmarDepartment of Veterinary Medicine, University of Cambridge, Cambridge, UK.
Miha PipanBetter Origin, Future Business Centre, Cambridge, UK.
Graham ChristieDepartment of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK.
Andrew J GrantDepartment of Veterinary Medicine, University of Cambridge, Cambridge, UK. ajg60@cam.ac.uk.

Funding

BBSRC BB/M011194/1
6 · The paper itself

Abstract

backgroundThe world faces a major infectious disease challenge. Interest in the discovery, design, or development of antimicrobial peptides (AMPs) as an alternative approach for the treatment of bacterial infections has increased. Insects are a good source of AMPs which are the main effector molecules of their innate immune system. Black Soldier Fly Larvae (BSFL) are being developed for large-scale rearing for food sustainability, waste reduction and as sustainable animal and fish feed. Bioinformatic studies have suggested that BSFL have the largest number of AMPs identified in insects. However, most AMPs identified in BSF have not yet undergone antimicrobial evaluation but are promising leads to treat critical infections.

resultsJg7197.t1, Jg7902.t1 and Jg7904.t1 were expressed into the haemolymph of larvae following infection with Salmonella enterica serovar Typhimurium and were predicted to be AMPs using the computational tool ampir. The genes encoding these proteins were within 2 distinct clusters in chromosome 1 of the BSF genome. Following removal of signal peptides, predicted structures of the mature proteins were superimposed, highlighting a high degree of structural conservation. The 3 AMPs share primary sequences with proteins that contain a Kunitz-binding domain; characterised for inhibitory action against proteases, and antimicrobial activities. An in vitro antimicrobial screen indicated that heterologously expressed SUMO-Jg7197.t1 and SUMO-Jg7902.t1 did not show activity against 12 bacterial strains. While recombinant SUMO-Jg7904.t1 had antimicrobial activity against a range of Gram-negative and Gram-positive bacteria, including the serious pathogen Pseudomonas aeruginosa.

conclusionsWe have cloned and purified putative AMPs from BSFL and performed initial in vitro experiments to evaluate their antimicrobial activity. In doing so, we have identified a putative novel defensin-like AMP, Jg7904.t1, encoded in a paralogous gene cluster, with antimicrobial activity against P. aeruginosa.

Indexed as

Anti-Bacterial AgentsDefensinsDipteraLarvaAmino Acid SequenceAnimalsAntimicrobial PeptidesGram-Negative BacteriaInsect ProteinsMicrobial Sensitivity TestsSalmonella typhimuriumAnti-Bacterial AgentsAntimicrobial PeptidesDefensinsInsect ProteinsAlphaFoldAntibioticsAntimicrobial peptidesBlack soldier fly larvaeDefensinsHermetia illucensPseudomonas aeruginosa

Identifiers

PMID38755524
PMCPMC11097590

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.