Evidence map›Paper›PMID 38755134›Full record

ArticleNature communications2024

Ser/Leu-swapped cell-free translation system constructed with natural/in vitro transcribed-hybrid tRNA set.

Tomoshige Fujino, Ryogo Sonoda, Taito Higashinagata, Emi Mishiro-Sato, Keiko Kano, Hiroshi Murakami

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Reconstituting alternative life using the test-bed of cell-free systems.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025
    Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Tomoshige FujinoDepartment of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Japan.ORCID http://orcid.org/0000-0002-1908-8757
Ryogo SonodaDepartment of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Japan.ORCID http://orcid.org/0009-0007-3921-1939
Taito HigashinagataDepartment of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Japan.ORCID http://orcid.org/0009-0008-8914-6589
Emi Mishiro-SatoInstitute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Nagoya, Japan.ORCID http://orcid.org/0000-0001-5528-4440
Keiko KanoInstitute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Nagoya, Japan.ORCID http://orcid.org/0000-0002-8535-9674
Hiroshi MurakamiDepartment of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Japan. murah@chembio.nagoya-u.ac.jp.ORCID http://orcid.org/0000-0003-2089-9263

Funding

MEXT | Japan Society for the Promotion of Science (JSPS) 21K05270MEXT | Japan Society for the Promotion of Science (JSPS) 23H05456
6 · The paper itself

Abstract

The Ser/Leu-swapped genetic code can act as a genetic firewall, mitigating biohazard risks arising from horizontal gene transfer in genetically modified organisms. Our prior work demonstrated the orthogonality of this swapped code to the standard genetic code using a cell-free translation system comprised of 21 in vitro transcribed tRNAs. In this study, to advance this system for protein engineering, we introduce a natural/in vitro transcribed-hybrid tRNA set. This set combines natural tRNAs from Escherichia coli (excluding Ser, Leu, and Tyr) and in vitro transcribed tRNAs, encompassing anticodon-swapped tRNA

Indexed as

Cell-Free SystemEscherichia coliProtein BiosynthesisAnticodonGenetic CodeGreen Fluorescent ProteinsProtein EngineeringRNA, TransferRNA, Transfer, LeuRNA, Transfer, SerTranscription, GeneticAnticodonGreen Fluorescent ProteinsRNA, TransferRNA, Transfer, LeuRNA, Transfer, Ser

Identifiers

PMID38755134
PMCPMC11099018

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.